Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21715 and BPHYT_RS21720 overlap by 8 nucleotides BPHYT_RS21720 and BPHYT_RS21725 are separated by 33 nucleotides BPHYT_RS21725 and BPHYT_RS21730 are separated by 345 nucleotides
BPHYT_RS21715: BPHYT_RS21715 - taurine ABC transporter permease, at 428,227 to 429,147
_RS21715
BPHYT_RS21720: BPHYT_RS21720 - nitrate ABC transporter ATP-binding protein, at 429,140 to 429,955
_RS21720
BPHYT_RS21725: BPHYT_RS21725 - taurine ABC transporter substrate-binding protein, at 429,989 to 431,023
_RS21725
BPHYT_RS21730: BPHYT_RS21730 - 2-aminoadipate aminotransferase, at 431,369 to 432,895
_RS21730
Position (kb) 429
430
431
432 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 429.135 kb on + strand at 429.189 kb on + strand at 429.298 kb on + strand, within BPHYT_RS21720 at 429.298 kb on + strand, within BPHYT_RS21720 at 429.552 kb on + strand, within BPHYT_RS21720 at 429.552 kb on + strand, within BPHYT_RS21720 at 429.552 kb on + strand, within BPHYT_RS21720 at 429.739 kb on + strand, within BPHYT_RS21720 at 429.985 kb on + strand at 430.017 kb on + strand at 430.018 kb on - strand at 430.163 kb on - strand, within BPHYT_RS21725 at 430.908 kb on + strand, within BPHYT_RS21725 at 431.050 kb on + strand at 431.062 kb on + strand at 431.214 kb on - strand at 431.223 kb on + strand at 431.223 kb on + strand at 431.223 kb on + strand at 431.594 kb on + strand, within BPHYT_RS21730 at 431.860 kb on + strand, within BPHYT_RS21730 at 431.861 kb on - strand, within BPHYT_RS21730
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 429,135 + +1.2 429,189 + -0.4 429,298 + BPHYT_RS21720 0.19 +0.2 429,298 + BPHYT_RS21720 0.19 -1.3 429,552 + BPHYT_RS21720 0.50 -0.6 429,552 + BPHYT_RS21720 0.50 +0.3 429,552 + BPHYT_RS21720 0.50 +0.2 429,739 + BPHYT_RS21720 0.73 -0.2 429,985 + -1.1 430,017 + -2.8 430,018 - +0.3 430,163 - BPHYT_RS21725 0.17 -2.8 430,908 + BPHYT_RS21725 0.89 -1.3 431,050 + -0.1 431,062 + -0.1 431,214 - -0.3 431,223 + +1.6 431,223 + -2.0 431,223 + +1.1 431,594 + BPHYT_RS21730 0.15 -0.9 431,860 + BPHYT_RS21730 0.32 +0.2 431,861 - BPHYT_RS21730 0.32 -0.3
Or see this region's nucleotide sequence