Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21320 and BPHYT_RS21325 are separated by 229 nucleotides BPHYT_RS21325 and BPHYT_RS21330 are separated by 384 nucleotides BPHYT_RS21330 and BPHYT_RS21335 are separated by 246 nucleotides BPHYT_RS21335 and BPHYT_RS21340 overlap by 4 nucleotides
BPHYT_RS21320: BPHYT_RS21320 - alkaline phosphatase, at 342,154 to 344,106
_RS21320
BPHYT_RS21325: BPHYT_RS21325 - hypothetical protein, at 344,336 to 344,674
_RS21325
BPHYT_RS21330: BPHYT_RS21330 - hypothetical protein, at 345,059 to 345,493
_RS21330
BPHYT_RS21335: BPHYT_RS21335 - CDP-alcohol phosphatidyltransferase, at 345,740 to 346,441
_RS21335
BPHYT_RS21340: BPHYT_RS21340 - 2OG-Fe(II) oxygenase, at 346,438 to 347,250
_RS21340
Position (kb) 345
346 Strain fitness (log2 ratio) -2
-1
0
1
2 at 344.104 kb on + strand at 344.104 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.234 kb on + strand at 344.235 kb on - strand at 344.237 kb on - strand at 344.237 kb on - strand at 344.259 kb on - strand at 344.314 kb on + strand at 344.353 kb on + strand at 344.474 kb on + strand, within BPHYT_RS21325 at 344.531 kb on + strand, within BPHYT_RS21325 at 344.531 kb on + strand, within BPHYT_RS21325 at 344.663 kb on + strand at 344.748 kb on + strand at 344.812 kb on + strand at 344.812 kb on + strand at 344.868 kb on - strand at 344.943 kb on + strand at 344.943 kb on + strand at 344.944 kb on - strand at 344.984 kb on + strand at 344.984 kb on + strand at 344.984 kb on + strand at 344.985 kb on - strand at 344.987 kb on - strand at 344.994 kb on - strand at 345.009 kb on + strand at 345.009 kb on + strand at 345.009 kb on + strand at 345.009 kb on + strand at 345.010 kb on - strand at 345.010 kb on - strand at 345.086 kb on - strand at 345.270 kb on + strand, within BPHYT_RS21330 at 345.429 kb on + strand, within BPHYT_RS21330 at 345.494 kb on - strand at 345.521 kb on + strand at 345.521 kb on + strand at 345.522 kb on - strand at 345.612 kb on + strand at 345.612 kb on + strand at 345.612 kb on + strand at 345.654 kb on - strand at 345.724 kb on + strand at 345.724 kb on + strand at 345.724 kb on + strand at 345.727 kb on - strand at 345.727 kb on - strand at 345.728 kb on + strand at 345.728 kb on + strand at 345.728 kb on + strand at 345.970 kb on + strand, within BPHYT_RS21335 at 346.443 kb on + strand at 346.482 kb on - strand at 346.482 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 344,104 + +0.1 344,104 + -0.5 344,234 + -2.0 344,234 + -0.5 344,234 + +0.4 344,234 + -2.3 344,234 + +0.9 344,234 + +0.9 344,235 - -0.6 344,237 - -0.5 344,237 - +1.4 344,259 - -1.3 344,314 + +0.0 344,353 + -1.9 344,474 + BPHYT_RS21325 0.41 +1.1 344,531 + BPHYT_RS21325 0.58 -1.7 344,531 + BPHYT_RS21325 0.58 -0.8 344,663 + +0.0 344,748 + +0.2 344,812 + -1.4 344,812 + +0.5 344,868 - +0.1 344,943 + -0.4 344,943 + -1.8 344,944 - +0.2 344,984 + +1.2 344,984 + -2.3 344,984 + -0.4 344,985 - -0.8 344,987 - +0.2 344,994 - +1.1 345,009 + +0.4 345,009 + +0.3 345,009 + -0.1 345,009 + +0.1 345,010 - -1.1 345,010 - -1.5 345,086 - -0.8 345,270 + BPHYT_RS21330 0.49 +2.1 345,429 + BPHYT_RS21330 0.85 -1.4 345,494 - +0.5 345,521 + -0.5 345,521 + +0.7 345,522 - -1.2 345,612 + -0.6 345,612 + -1.4 345,612 + -1.4 345,654 - -1.2 345,724 + +0.2 345,724 + -2.1 345,724 + -1.4 345,727 - -0.3 345,727 - -0.3 345,728 + -0.4 345,728 + -0.3 345,728 + +0.1 345,970 + BPHYT_RS21335 0.33 -1.8 346,443 + +0.3 346,482 - +1.9 346,482 - +1.9
Or see this region's nucleotide sequence