Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21260 and BPHYT_RS21265 overlap by 1 nucleotides BPHYT_RS21265 and BPHYT_RS21270 are separated by 57 nucleotides BPHYT_RS21270 and BPHYT_RS21275 are separated by 105 nucleotides BPHYT_RS21275 and BPHYT_RS21280 are separated by 216 nucleotides
BPHYT_RS21260: BPHYT_RS21260 - ABC transporter permease, at 330,662 to 331,540
_RS21260
BPHYT_RS21265: BPHYT_RS21265 - ABC transporter permease, at 331,540 to 332,478
_RS21265
BPHYT_RS21270: BPHYT_RS21270 - dihydroxy-acid dehydratase, at 332,536 to 334,296
_RS21270
BPHYT_RS21275: BPHYT_RS21275 - short-chain dehydrogenase, at 334,402 to 335,007
_RS21275
BPHYT_RS21280: BPHYT_RS21280 - hypothetical protein, at 335,224 to 335,676
_RS21280
Position (kb)
332
333
334
335 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 331.807 kb on + strand, within BPHYT_RS21265 at 331.808 kb on - strand, within BPHYT_RS21265 at 332.026 kb on + strand, within BPHYT_RS21265 at 332.027 kb on - strand, within BPHYT_RS21265 at 332.027 kb on - strand, within BPHYT_RS21265 at 332.027 kb on - strand, within BPHYT_RS21265 at 332.027 kb on - strand, within BPHYT_RS21265 at 332.191 kb on + strand, within BPHYT_RS21265 at 332.323 kb on + strand, within BPHYT_RS21265 at 332.323 kb on + strand, within BPHYT_RS21265 at 332.324 kb on - strand, within BPHYT_RS21265 at 332.326 kb on + strand, within BPHYT_RS21265 at 332.326 kb on + strand, within BPHYT_RS21265 at 332.326 kb on + strand, within BPHYT_RS21265 at 332.386 kb on + strand at 332.386 kb on + strand at 332.387 kb on - strand at 332.387 kb on - strand at 332.388 kb on + strand at 332.654 kb on - strand at 333.378 kb on + strand, within BPHYT_RS21270 at 333.485 kb on - strand, within BPHYT_RS21270 at 333.779 kb on - strand, within BPHYT_RS21270 at 333.779 kb on - strand, within BPHYT_RS21270 at 333.896 kb on + strand, within BPHYT_RS21270 at 333.977 kb on - strand, within BPHYT_RS21270 at 334.273 kb on - strand at 334.514 kb on + strand, within BPHYT_RS21275 at 334.515 kb on - strand, within BPHYT_RS21275 at 334.515 kb on - strand, within BPHYT_RS21275 at 334.706 kb on + strand, within BPHYT_RS21275 at 334.707 kb on - strand, within BPHYT_RS21275 at 335.009 kb on + strand at 335.010 kb on - strand at 335.010 kb on - strand at 335.157 kb on - strand at 335.166 kb on - strand at 335.166 kb on - strand at 335.272 kb on - strand, within BPHYT_RS21280
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 331,807 + BPHYT_RS21265 0.28 +1.3 331,808 - BPHYT_RS21265 0.29 +0.7 332,026 + BPHYT_RS21265 0.52 -1.2 332,027 - BPHYT_RS21265 0.52 +0.0 332,027 - BPHYT_RS21265 0.52 +0.1 332,027 - BPHYT_RS21265 0.52 -0.7 332,027 - BPHYT_RS21265 0.52 -0.9 332,191 + BPHYT_RS21265 0.69 -1.7 332,323 + BPHYT_RS21265 0.83 -1.4 332,323 + BPHYT_RS21265 0.83 +0.2 332,324 - BPHYT_RS21265 0.83 -1.7 332,326 + BPHYT_RS21265 0.84 +0.2 332,326 + BPHYT_RS21265 0.84 +0.5 332,326 + BPHYT_RS21265 0.84 +0.9 332,386 + +0.6 332,386 + +0.5 332,387 - -2.7 332,387 - +0.9 332,388 + -0.2 332,654 - +0.2 333,378 + BPHYT_RS21270 0.48 -0.2 333,485 - BPHYT_RS21270 0.54 -0.4 333,779 - BPHYT_RS21270 0.71 -2.2 333,779 - BPHYT_RS21270 0.71 -0.7 333,896 + BPHYT_RS21270 0.77 +0.4 333,977 - BPHYT_RS21270 0.82 +0.1 334,273 - -3.0 334,514 + BPHYT_RS21275 0.18 +0.1 334,515 - BPHYT_RS21275 0.19 -0.0 334,515 - BPHYT_RS21275 0.19 -1.4 334,706 + BPHYT_RS21275 0.50 -0.7 334,707 - BPHYT_RS21275 0.50 +0.1 335,009 + +0.4 335,010 - -0.3 335,010 - +0.8 335,157 - -0.1 335,166 - +1.9 335,166 - +2.9 335,272 - BPHYT_RS21280 0.11 -0.8
Or see this region's nucleotide sequence