Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21050 and BPHYT_RS21055 are separated by 2 nucleotides BPHYT_RS21055 and BPHYT_RS21060 are separated by 202 nucleotides
BPHYT_RS21050: BPHYT_RS21050 - aldehyde-activating protein, at 283,325 to 283,900
_RS21050
BPHYT_RS21055: BPHYT_RS21055 - S-formylglutathione hydrolase, at 283,903 to 284,733
_RS21055
BPHYT_RS21060: BPHYT_RS21060 - hypothetical protein, at 284,936 to 286,408
_RS21060
Position (kb) 283
284
285 Strain fitness (log2 ratio) -2
-1
0
1
2 at 282.921 kb on + strand at 282.970 kb on + strand at 282.979 kb on + strand at 282.979 kb on + strand at 282.979 kb on + strand at 283.080 kb on + strand at 283.297 kb on + strand at 283.301 kb on - strand at 283.304 kb on - strand at 283.398 kb on + strand, within BPHYT_RS21050 at 283.398 kb on + strand, within BPHYT_RS21050 at 283.474 kb on + strand, within BPHYT_RS21050 at 283.632 kb on - strand, within BPHYT_RS21050 at 283.831 kb on - strand, within BPHYT_RS21050 at 284.007 kb on + strand, within BPHYT_RS21055 at 284.131 kb on - strand, within BPHYT_RS21055 at 284.264 kb on - strand, within BPHYT_RS21055 at 284.497 kb on + strand, within BPHYT_RS21055 at 284.668 kb on - strand at 284.668 kb on - strand at 284.675 kb on - strand at 284.810 kb on - strand at 285.011 kb on - strand at 285.257 kb on + strand, within BPHYT_RS21060 at 285.262 kb on - strand, within BPHYT_RS21060 at 285.294 kb on + strand, within BPHYT_RS21060 at 285.295 kb on - strand, within BPHYT_RS21060
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 282,921 + -0.1 282,970 + +1.3 282,979 + +0.4 282,979 + -1.5 282,979 + -2.0 283,080 + +0.4 283,297 + -1.1 283,301 - +1.3 283,304 - -0.3 283,398 + BPHYT_RS21050 0.13 +1.0 283,398 + BPHYT_RS21050 0.13 -1.1 283,474 + BPHYT_RS21050 0.26 +1.0 283,632 - BPHYT_RS21050 0.53 -0.3 283,831 - BPHYT_RS21050 0.88 +0.2 284,007 + BPHYT_RS21055 0.13 -1.2 284,131 - BPHYT_RS21055 0.27 -0.2 284,264 - BPHYT_RS21055 0.43 -0.1 284,497 + BPHYT_RS21055 0.71 -2.4 284,668 - +0.0 284,668 - +0.1 284,675 - +1.3 284,810 - +0.6 285,011 - +2.3 285,257 + BPHYT_RS21060 0.22 -0.1 285,262 - BPHYT_RS21060 0.22 +0.9 285,294 + BPHYT_RS21060 0.24 -0.2 285,295 - BPHYT_RS21060 0.24 -0.4
Or see this region's nucleotide sequence