Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20465 and BPHYT_RS20470 are separated by 55 nucleotides BPHYT_RS20470 and BPHYT_RS20475 are separated by 32 nucleotides BPHYT_RS20475 and BPHYT_RS20480 are separated by 107 nucleotides
BPHYT_RS20465: BPHYT_RS20465 - LysR family transcriptional regulator, at 153,559 to 154,443
_RS20465
BPHYT_RS20470: BPHYT_RS20470 - hypothetical protein, at 154,499 to 155,131
_RS20470
BPHYT_RS20475: BPHYT_RS20475 - hypothetical protein, at 155,164 to 156,384
_RS20475
BPHYT_RS20480: BPHYT_RS20480 - hypothetical protein, at 156,492 to 157,259
_RS20480
Position (kb) 155
156
157 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 154.361 kb on - strand at 154.551 kb on + strand at 154.552 kb on - strand at 154.552 kb on - strand at 154.907 kb on - strand, within BPHYT_RS20470 at 155.192 kb on - strand at 155.524 kb on + strand, within BPHYT_RS20475 at 155.974 kb on - strand, within BPHYT_RS20475 at 156.443 kb on + strand at 156.711 kb on + strand, within BPHYT_RS20480 at 156.712 kb on - strand, within BPHYT_RS20480 at 156.861 kb on + strand, within BPHYT_RS20480 at 156.861 kb on + strand, within BPHYT_RS20480 at 156.862 kb on - strand, within BPHYT_RS20480 at 156.862 kb on - strand, within BPHYT_RS20480 at 156.975 kb on + strand, within BPHYT_RS20480 at 156.975 kb on + strand, within BPHYT_RS20480 at 156.982 kb on - strand, within BPHYT_RS20480 at 156.982 kb on - strand, within BPHYT_RS20480 at 156.982 kb on - strand, within BPHYT_RS20480 at 156.982 kb on - strand, within BPHYT_RS20480 at 157.071 kb on + strand, within BPHYT_RS20480 at 157.072 kb on - strand, within BPHYT_RS20480 at 157.134 kb on + strand, within BPHYT_RS20480
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 154,361 - -0.7 154,551 + -1.0 154,552 - +0.0 154,552 - -1.4 154,907 - BPHYT_RS20470 0.64 +0.2 155,192 - -0.3 155,524 + BPHYT_RS20475 0.29 -1.7 155,974 - BPHYT_RS20475 0.66 -1.6 156,443 + -0.3 156,711 + BPHYT_RS20480 0.29 -1.4 156,712 - BPHYT_RS20480 0.29 +0.7 156,861 + BPHYT_RS20480 0.48 -0.8 156,861 + BPHYT_RS20480 0.48 -1.3 156,862 - BPHYT_RS20480 0.48 +0.3 156,862 - BPHYT_RS20480 0.48 -0.4 156,975 + BPHYT_RS20480 0.63 -0.3 156,975 + BPHYT_RS20480 0.63 +0.8 156,982 - BPHYT_RS20480 0.64 -2.8 156,982 - BPHYT_RS20480 0.64 -0.4 156,982 - BPHYT_RS20480 0.64 +0.6 156,982 - BPHYT_RS20480 0.64 -0.4 157,071 + BPHYT_RS20480 0.75 -1.9 157,072 - BPHYT_RS20480 0.76 +1.4 157,134 + BPHYT_RS20480 0.84 +1.9
Or see this region's nucleotide sequence