Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20405 and BPHYT_RS20410 are separated by 132 nucleotides BPHYT_RS20410 and BPHYT_RS20415 are separated by 76 nucleotides
BPHYT_RS20405: BPHYT_RS20405 - MFS transporter, at 140,181 to 141,512
_RS20405
BPHYT_RS20410: BPHYT_RS20410 - glucarate dehydratase, at 141,645 to 142,994
_RS20410
BPHYT_RS20415: BPHYT_RS20415 - 5-dehydro-4-deoxyglucarate dehydratase, at 143,071 to 143,988
_RS20415
Position (kb) 141
142
143 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 140.758 kb on + strand, within BPHYT_RS20405 at 140.795 kb on + strand, within BPHYT_RS20405 at 140.796 kb on - strand, within BPHYT_RS20405 at 140.949 kb on + strand, within BPHYT_RS20405 at 140.949 kb on + strand, within BPHYT_RS20405 at 140.973 kb on + strand, within BPHYT_RS20405 at 141.084 kb on + strand, within BPHYT_RS20405 at 141.084 kb on + strand, within BPHYT_RS20405 at 141.085 kb on - strand, within BPHYT_RS20405 at 141.085 kb on - strand, within BPHYT_RS20405 at 141.514 kb on + strand at 141.514 kb on + strand at 141.624 kb on + strand at 141.625 kb on - strand at 141.735 kb on - strand at 141.849 kb on - strand, within BPHYT_RS20410 at 142.104 kb on + strand, within BPHYT_RS20410 at 142.104 kb on + strand, within BPHYT_RS20410 at 142.105 kb on - strand, within BPHYT_RS20410 at 142.114 kb on - strand, within BPHYT_RS20410 at 142.146 kb on + strand, within BPHYT_RS20410 at 142.893 kb on + strand at 142.941 kb on + strand at 142.941 kb on + strand at 142.942 kb on - strand at 143.170 kb on + strand, within BPHYT_RS20415 at 143.170 kb on + strand, within BPHYT_RS20415 at 143.171 kb on - strand, within BPHYT_RS20415 at 143.497 kb on + strand, within BPHYT_RS20415 at 143.497 kb on + strand, within BPHYT_RS20415 at 143.498 kb on - strand, within BPHYT_RS20415 at 143.671 kb on + strand, within BPHYT_RS20415 at 143.685 kb on + strand, within BPHYT_RS20415 at 143.706 kb on - strand, within BPHYT_RS20415 at 143.707 kb on + strand, within BPHYT_RS20415 at 143.708 kb on - strand, within BPHYT_RS20415 at 143.851 kb on + strand, within BPHYT_RS20415
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 140,758 + BPHYT_RS20405 0.43 -0.2 140,795 + BPHYT_RS20405 0.46 -0.4 140,796 - BPHYT_RS20405 0.46 -1.4 140,949 + BPHYT_RS20405 0.58 +0.3 140,949 + BPHYT_RS20405 0.58 -1.3 140,973 + BPHYT_RS20405 0.59 -1.5 141,084 + BPHYT_RS20405 0.68 -1.5 141,084 + BPHYT_RS20405 0.68 +0.2 141,085 - BPHYT_RS20405 0.68 -0.7 141,085 - BPHYT_RS20405 0.68 +1.4 141,514 + +0.9 141,514 + +0.7 141,624 + +0.6 141,625 - -0.0 141,735 - -0.3 141,849 - BPHYT_RS20410 0.15 -0.3 142,104 + BPHYT_RS20410 0.34 -0.6 142,104 + BPHYT_RS20410 0.34 -3.1 142,105 - BPHYT_RS20410 0.34 -2.4 142,114 - BPHYT_RS20410 0.35 -0.1 142,146 + BPHYT_RS20410 0.37 -2.9 142,893 + +0.2 142,941 + -0.3 142,941 + +0.3 142,942 - +1.8 143,170 + BPHYT_RS20415 0.11 +2.3 143,170 + BPHYT_RS20415 0.11 -1.6 143,171 - BPHYT_RS20415 0.11 -1.4 143,497 + BPHYT_RS20415 0.46 -0.9 143,497 + BPHYT_RS20415 0.46 -0.3 143,498 - BPHYT_RS20415 0.47 -0.6 143,671 + BPHYT_RS20415 0.65 -1.2 143,685 + BPHYT_RS20415 0.67 +0.4 143,706 - BPHYT_RS20415 0.69 +0.1 143,707 + BPHYT_RS20415 0.69 -1.6 143,708 - BPHYT_RS20415 0.69 +1.9 143,851 + BPHYT_RS20415 0.85 -0.7
Or see this region's nucleotide sequence