Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18900 and BPHYT_RS18905 are separated by 201 nucleotides BPHYT_RS18905 and BPHYT_RS18910 are separated by 94 nucleotides BPHYT_RS18910 and BPHYT_RS18915 are separated by 308 nucleotides BPHYT_RS18915 and BPHYT_RS18920 overlap by 4 nucleotides BPHYT_RS18920 and BPHYT_RS18925 are separated by 60 nucleotides
BPHYT_RS18900: BPHYT_RS18900 - flagella basal body P-ring formation protein FlgA, at 4,246,027 to 4,247,541
_RS18900
BPHYT_RS18905: BPHYT_RS18905 - anti-sigma-28 factor FlgM, at 4,247,743 to 4,248,096
_RS18905
BPHYT_RS18910: BPHYT_RS18910 - FlgN, at 4,248,191 to 4,248,637
_RS18910
BPHYT_RS18915: BPHYT_RS18915 - hypothetical protein, at 4,248,946 to 4,249,128
_RS18915
BPHYT_RS18920: BPHYT_RS18920 - hypothetical protein, at 4,249,125 to 4,249,535
_RS18920
BPHYT_RS18925: BPHYT_RS18925 - RNA polymerase sigma70, at 4,249,596 to 4,250,330
_RS18925
Position (kb)
4248
4249 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4247.656 kb on + strand at 4247.656 kb on + strand at 4247.657 kb on - strand at 4247.657 kb on - strand at 4247.657 kb on - strand at 4247.666 kb on + strand at 4247.712 kb on + strand at 4247.714 kb on + strand at 4247.714 kb on + strand at 4247.714 kb on + strand at 4248.111 kb on + strand at 4248.503 kb on - strand, within BPHYT_RS18910 at 4248.539 kb on + strand, within BPHYT_RS18910 at 4248.540 kb on - strand, within BPHYT_RS18910 at 4248.596 kb on + strand at 4248.597 kb on - strand at 4248.775 kb on - strand at 4248.837 kb on + strand at 4248.837 kb on + strand at 4248.838 kb on - strand at 4248.896 kb on + strand at 4248.945 kb on + strand at 4249.411 kb on + strand, within BPHYT_RS18920 at 4249.411 kb on + strand, within BPHYT_RS18920 at 4249.415 kb on + strand, within BPHYT_RS18920 at 4249.415 kb on + strand, within BPHYT_RS18920 at 4249.421 kb on + strand, within BPHYT_RS18920 at 4249.421 kb on + strand, within BPHYT_RS18920 at 4249.461 kb on + strand, within BPHYT_RS18920
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 4,247,656 + -0.4 4,247,656 + +0.1 4,247,657 - -0.9 4,247,657 - -0.5 4,247,657 - -2.4 4,247,666 + +0.1 4,247,712 + -0.2 4,247,714 + -0.2 4,247,714 + -0.2 4,247,714 + +0.3 4,248,111 + -1.1 4,248,503 - BPHYT_RS18910 0.70 -1.1 4,248,539 + BPHYT_RS18910 0.78 -1.1 4,248,540 - BPHYT_RS18910 0.78 -0.7 4,248,596 + +0.6 4,248,597 - +0.2 4,248,775 - -0.9 4,248,837 + +2.5 4,248,837 + -0.2 4,248,838 - -0.0 4,248,896 + +0.6 4,248,945 + -1.6 4,249,411 + BPHYT_RS18920 0.70 -2.6 4,249,411 + BPHYT_RS18920 0.70 -0.3 4,249,415 + BPHYT_RS18920 0.71 -3.0 4,249,415 + BPHYT_RS18920 0.71 +0.2 4,249,421 + BPHYT_RS18920 0.72 +0.1 4,249,421 + BPHYT_RS18920 0.72 -3.3 4,249,461 + BPHYT_RS18920 0.82 +0.5
Or see this region's nucleotide sequence