Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14745 and BPHYT_RS14750 are separated by 93 nucleotides BPHYT_RS14750 and BPHYT_RS14755 are separated by 145 nucleotides BPHYT_RS14755 and BPHYT_RS14760 are separated by 25 nucleotides BPHYT_RS14760 and BPHYT_RS14765 are separated by 132 nucleotides
BPHYT_RS14745: BPHYT_RS14745 - DNA mismatch repair protein MutT, at 3,357,507 to 3,358,235
_RS14745
BPHYT_RS14750: BPHYT_RS14750 - 50S ribosomal protein L19, at 3,358,329 to 3,358,718
_RS14750
BPHYT_RS14755: BPHYT_RS14755 - tRNA (guanine-N(1)-)-methyltransferase, at 3,358,864 to 3,359,631
_RS14755
BPHYT_RS14760: BPHYT_RS14760 - ribosome maturation factor RimM, at 3,359,657 to 3,360,403
_RS14760
BPHYT_RS14765: BPHYT_RS14765 - 30S ribosomal protein S16, at 3,360,536 to 3,360,790
_RS14765
Position (kb)
3358
3359
3360 Strain fitness (log2 ratio)
-1
0
1
2 at 3358.321 kb on + strand at 3359.061 kb on - strand, within BPHYT_RS14755 at 3359.079 kb on - strand, within BPHYT_RS14755 at 3359.496 kb on - strand, within BPHYT_RS14755 at 3359.530 kb on - strand, within BPHYT_RS14755
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 3,358,321 + +2.0 3,359,061 - BPHYT_RS14755 0.26 +2.0 3,359,079 - BPHYT_RS14755 0.28 -0.0 3,359,496 - BPHYT_RS14755 0.82 -0.7 3,359,530 - BPHYT_RS14755 0.87 +0.7
Or see this region's nucleotide sequence