Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14170 and BPHYT_RS14175 are separated by 183 nucleotides BPHYT_RS14175 and BPHYT_RS14180 are separated by 145 nucleotides BPHYT_RS14180 and BPHYT_RS14185 are separated by 20 nucleotides BPHYT_RS14185 and BPHYT_RS14190 are separated by 151 nucleotides
BPHYT_RS14170: BPHYT_RS14170 - ribosomal RNA large subunit methyltransferase E, at 3,237,018 to 3,237,680
_RS14170
BPHYT_RS14175: BPHYT_RS14175 - RNA-binding protein, at 3,237,864 to 3,238,391
_RS14175
BPHYT_RS14180: BPHYT_RS14180 - membrane protein, at 3,238,537 to 3,239,046
_RS14180
BPHYT_RS14185: BPHYT_RS14185 - transcription elongation factor GreA, at 3,239,067 to 3,239,543
_RS14185
BPHYT_RS14190: BPHYT_RS14190 - carbamoyl phosphate synthase large subunit, at 3,239,695 to 3,242,949
_RS14190
Position (kb)
3238
3239
3240 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3237.717 kb on - strand at 3237.724 kb on - strand at 3238.059 kb on + strand, within BPHYT_RS14175 at 3238.060 kb on - strand, within BPHYT_RS14175 at 3238.060 kb on - strand, within BPHYT_RS14175 at 3238.103 kb on + strand, within BPHYT_RS14175 at 3238.104 kb on - strand, within BPHYT_RS14175 at 3238.390 kb on - strand at 3238.502 kb on + strand at 3238.503 kb on - strand at 3238.649 kb on + strand, within BPHYT_RS14180 at 3238.695 kb on - strand, within BPHYT_RS14180 at 3238.753 kb on - strand, within BPHYT_RS14180 at 3238.983 kb on - strand, within BPHYT_RS14180 at 3239.074 kb on + strand at 3239.074 kb on + strand at 3239.075 kb on - strand at 3239.095 kb on + strand at 3239.231 kb on - strand, within BPHYT_RS14185 at 3239.544 kb on - strand at 3239.554 kb on - strand at 3239.554 kb on - strand at 3239.590 kb on - strand at 3239.657 kb on - strand at 3239.669 kb on + strand at 3239.670 kb on - strand at 3239.670 kb on - strand at 3239.696 kb on + strand at 3239.722 kb on + strand at 3239.796 kb on - strand at 3239.798 kb on + strand at 3239.799 kb on - strand at 3239.799 kb on - strand at 3239.939 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 3,237,717 - +0.4 3,237,724 - -0.6 3,238,059 + BPHYT_RS14175 0.37 -2.4 3,238,060 - BPHYT_RS14175 0.37 -0.1 3,238,060 - BPHYT_RS14175 0.37 -0.5 3,238,103 + BPHYT_RS14175 0.45 +1.1 3,238,104 - BPHYT_RS14175 0.45 -0.1 3,238,390 - -1.0 3,238,502 + +1.3 3,238,503 - +1.0 3,238,649 + BPHYT_RS14180 0.22 -0.8 3,238,695 - BPHYT_RS14180 0.31 +2.8 3,238,753 - BPHYT_RS14180 0.42 +1.0 3,238,983 - BPHYT_RS14180 0.87 -1.0 3,239,074 + -0.0 3,239,074 + +0.3 3,239,075 - +0.5 3,239,095 + +2.5 3,239,231 - BPHYT_RS14185 0.34 -0.7 3,239,544 - -0.9 3,239,554 - +0.6 3,239,554 - +0.3 3,239,590 - -1.1 3,239,657 - +1.1 3,239,669 + +1.0 3,239,670 - -0.0 3,239,670 - -2.1 3,239,696 + +1.5 3,239,722 + +0.5 3,239,796 - +2.5 3,239,798 + +1.5 3,239,799 - +2.5 3,239,799 - +0.2 3,239,939 - -0.6
Or see this region's nucleotide sequence