Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13460 and BPHYT_RS13465 are separated by 45 nucleotides BPHYT_RS13465 and BPHYT_RS13470 are separated by 94 nucleotides BPHYT_RS13470 and BPHYT_RS13475 are separated by 155 nucleotides
BPHYT_RS13460: BPHYT_RS13460 - chemotaxis protein, at 3,072,818 to 3,074,398
_RS13460
BPHYT_RS13465: BPHYT_RS13465 - hypothetical protein, at 3,074,444 to 3,074,824
_RS13465
BPHYT_RS13470: BPHYT_RS13470 - arylsulfate sulfotransferase, at 3,074,919 to 3,075,269
_RS13470
BPHYT_RS13475: BPHYT_RS13475 - 2-keto-gluconate dehydrogenase, at 3,075,425 to 3,077,011
_RS13475
Position (kb) 3074
3075 Strain fitness (log2 ratio) -2
-1
0
1 at 3074.153 kb on - strand, within BPHYT_RS13460 at 3074.423 kb on + strand at 3074.563 kb on - strand, within BPHYT_RS13465 at 3074.631 kb on + strand, within BPHYT_RS13465 at 3074.632 kb on - strand, within BPHYT_RS13465 at 3074.765 kb on - strand, within BPHYT_RS13465 at 3074.839 kb on + strand at 3075.022 kb on + strand, within BPHYT_RS13470 at 3075.257 kb on - strand at 3075.259 kb on - strand at 3075.350 kb on + strand at 3075.688 kb on - strand, within BPHYT_RS13475
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 3,074,153 - BPHYT_RS13460 0.84 -0.2 3,074,423 + -2.6 3,074,563 - BPHYT_RS13465 0.31 +0.4 3,074,631 + BPHYT_RS13465 0.49 -1.0 3,074,632 - BPHYT_RS13465 0.49 +0.6 3,074,765 - BPHYT_RS13465 0.84 -1.1 3,074,839 + +0.2 3,075,022 + BPHYT_RS13470 0.29 -0.6 3,075,257 - -0.6 3,075,259 - +0.8 3,075,350 + +0.9 3,075,688 - BPHYT_RS13475 0.17 +0.3
Or see this region's nucleotide sequence