Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12550

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12545 and BPHYT_RS12550 are separated by 49 nucleotidesBPHYT_RS12550 and BPHYT_RS12555 are separated by 72 nucleotidesBPHYT_RS12555 and BPHYT_RS12560 are separated by 153 nucleotides BPHYT_RS12545: BPHYT_RS12545 - membrane protein, at 2,863,448 to 2,864,848 _RS12545 BPHYT_RS12550: BPHYT_RS12550 - GTP-binding protein HflX, at 2,864,898 to 2,866,070 _RS12550 BPHYT_RS12555: BPHYT_RS12555 - RNA-binding protein Hfq, at 2,866,143 to 2,866,379 _RS12555 BPHYT_RS12560: BPHYT_RS12560 - GTPase Der, at 2,866,533 to 2,867,870 _RS12560 Position (kb) 2864 2865 2866 2867Strain fitness (log2 ratio) -2 -1 0 1at 2865.080 kb on - strand, within BPHYT_RS12550at 2865.967 kb on - strandat 2866.535 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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2,865,080 - BPHYT_RS12550 0.16 -2.2
2,865,967 - -0.3
2,866,535 - +1.6

Or see this region's nucleotide sequence