Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11995

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11990 and BPHYT_RS11995 overlap by 4 nucleotidesBPHYT_RS11995 and BPHYT_RS12000 are separated by 140 nucleotides BPHYT_RS11990: BPHYT_RS11990 - hypothetical protein, at 2,728,832 to 2,729,575 _RS11990 BPHYT_RS11995: BPHYT_RS11995 - flagellar hook protein FlgE, at 2,729,572 to 2,730,804 _RS11995 BPHYT_RS12000: BPHYT_RS12000 - sugar ABC transporter substrate-binding protein, at 2,730,945 to 2,732,000 _RS12000 Position (kb) 2729 2730 2731Strain fitness (log2 ratio) -2 -1 0 1at 2728.601 kb on + strandat 2728.602 kb on - strandat 2728.609 kb on + strandat 2728.782 kb on + strandat 2728.790 kb on + strandat 2728.790 kb on + strandat 2728.791 kb on - strandat 2728.791 kb on - strandat 2728.928 kb on - strand, within BPHYT_RS11990at 2729.245 kb on + strand, within BPHYT_RS11990at 2729.500 kb on - strand, within BPHYT_RS11990at 2729.811 kb on - strand, within BPHYT_RS11995at 2730.506 kb on - strand, within BPHYT_RS11995at 2730.525 kb on - strand, within BPHYT_RS11995at 2730.595 kb on + strand, within BPHYT_RS11995at 2730.800 kb on + strandat 2730.803 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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2,728,601 + -0.7
2,728,602 - +0.4
2,728,609 + -2.2
2,728,782 + -0.3
2,728,790 + +0.9
2,728,790 + +0.3
2,728,791 - +0.7
2,728,791 - -0.1
2,728,928 - BPHYT_RS11990 0.13 +0.5
2,729,245 + BPHYT_RS11990 0.56 -0.7
2,729,500 - BPHYT_RS11990 0.90 +0.3
2,729,811 - BPHYT_RS11995 0.19 -0.8
2,730,506 - BPHYT_RS11995 0.76 -0.3
2,730,525 - BPHYT_RS11995 0.77 -1.5
2,730,595 + BPHYT_RS11995 0.83 -0.8
2,730,800 + -0.8
2,730,803 - -1.4

Or see this region's nucleotide sequence