Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11225 and BPHYT_RS11230 are separated by 208 nucleotides BPHYT_RS11230 and BPHYT_RS11235 are separated by 136 nucleotides BPHYT_RS11235 and BPHYT_RS11240 are separated by 18 nucleotides BPHYT_RS11240 and BPHYT_RS11245 overlap by 4 nucleotides BPHYT_RS11245 and BPHYT_RS11250 are separated by 151 nucleotides
BPHYT_RS11225: BPHYT_RS11225 - magnesium transporter, at 2,554,018 to 2,554,995
_RS11225
BPHYT_RS11230: BPHYT_RS11230 - hypothetical protein, at 2,555,204 to 2,555,680
_RS11230
BPHYT_RS11235: BPHYT_RS11235 - hypothetical protein, at 2,555,817 to 2,556,050
_RS11235
BPHYT_RS11240: BPHYT_RS11240 - membrane protein, at 2,556,069 to 2,556,218
_RS11240
BPHYT_RS11245: BPHYT_RS11245 - ATPase, at 2,556,215 to 2,556,832
_RS11245
BPHYT_RS11250: BPHYT_RS11250 - saccharopine dehydrogenase, at 2,556,984 to 2,558,081
_RS11250
Position (kb)
2555
2556
2557 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2555.003 kb on + strand at 2555.036 kb on + strand at 2555.037 kb on - strand at 2555.042 kb on - strand at 2555.046 kb on - strand at 2555.094 kb on + strand at 2555.102 kb on - strand at 2555.130 kb on - strand at 2555.178 kb on - strand at 2555.186 kb on + strand at 2555.186 kb on + strand at 2555.206 kb on - strand at 2555.362 kb on - strand, within BPHYT_RS11230 at 2555.598 kb on - strand, within BPHYT_RS11230 at 2555.820 kb on + strand at 2555.821 kb on - strand at 2555.957 kb on + strand, within BPHYT_RS11235 at 2556.050 kb on + strand at 2556.051 kb on - strand at 2556.051 kb on - strand at 2556.585 kb on + strand, within BPHYT_RS11245 at 2556.762 kb on - strand, within BPHYT_RS11245 at 2556.762 kb on - strand, within BPHYT_RS11245 at 2556.851 kb on - strand at 2557.022 kb on - strand at 2557.022 kb on - strand at 2557.022 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,555,003 + +0.2 2,555,036 + +1.1 2,555,037 - +0.0 2,555,042 - +0.4 2,555,046 - +1.5 2,555,094 + -0.2 2,555,102 - -0.3 2,555,130 - -0.0 2,555,178 - +0.2 2,555,186 + -1.8 2,555,186 + +0.0 2,555,206 - -0.1 2,555,362 - BPHYT_RS11230 0.33 +0.2 2,555,598 - BPHYT_RS11230 0.83 +0.4 2,555,820 + -2.0 2,555,821 - -0.2 2,555,957 + BPHYT_RS11235 0.60 +2.3 2,556,050 + -1.2 2,556,051 - +1.5 2,556,051 - -1.5 2,556,585 + BPHYT_RS11245 0.60 -1.4 2,556,762 - BPHYT_RS11245 0.89 -1.2 2,556,762 - BPHYT_RS11245 0.89 -0.1 2,556,851 - -0.1 2,557,022 - -0.6 2,557,022 - -0.2 2,557,022 - +0.2
Or see this region's nucleotide sequence