Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11145 and BPHYT_RS11150 are separated by 251 nucleotides BPHYT_RS11150 and BPHYT_RS11155 are separated by 53 nucleotides BPHYT_RS11155 and BPHYT_RS11160 are separated by 174 nucleotides BPHYT_RS11160 and BPHYT_RS11165 are separated by 212 nucleotides
BPHYT_RS11145: BPHYT_RS11145 - short-chain dehydrogenase, at 2,533,046 to 2,533,819
_RS11145
BPHYT_RS11150: BPHYT_RS11150 - LysR family transcriptional regulator, at 2,534,071 to 2,535,000
_RS11150
BPHYT_RS11155: BPHYT_RS11155 - hypothetical protein, at 2,535,054 to 2,535,305
_RS11155
BPHYT_RS11160: BPHYT_RS11160 - hypothetical protein, at 2,535,480 to 2,535,659
_RS11160
BPHYT_RS11165: BPHYT_RS11165 - glycosyl transferase, at 2,535,872 to 2,536,954
_RS11165
Position (kb)
2534
2535
2536 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2533.847 kb on - strand at 2533.979 kb on - strand at 2533.982 kb on - strand at 2534.065 kb on + strand at 2534.065 kb on + strand at 2534.555 kb on + strand, within BPHYT_RS11150 at 2534.897 kb on - strand, within BPHYT_RS11150 at 2535.092 kb on - strand, within BPHYT_RS11155 at 2535.311 kb on - strand at 2535.663 kb on - strand at 2535.855 kb on + strand at 2535.871 kb on + strand at 2535.871 kb on + strand at 2535.871 kb on + strand at 2535.872 kb on - strand at 2535.983 kb on + strand, within BPHYT_RS11165
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,533,847 - -0.0 2,533,979 - -2.6 2,533,982 - +0.1 2,534,065 + -0.1 2,534,065 + -0.0 2,534,555 + BPHYT_RS11150 0.52 -3.1 2,534,897 - BPHYT_RS11150 0.89 +0.4 2,535,092 - BPHYT_RS11155 0.15 -0.5 2,535,311 - -0.3 2,535,663 - -1.4 2,535,855 + +0.5 2,535,871 + -0.8 2,535,871 + +0.2 2,535,871 + +0.2 2,535,872 - +0.0 2,535,983 + BPHYT_RS11165 0.10 -0.7
Or see this region's nucleotide sequence