Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10890 and BPHYT_RS10895 are separated by 179 nucleotides BPHYT_RS10895 and BPHYT_RS10900 overlap by 4 nucleotides BPHYT_RS10900 and BPHYT_RS10905 are separated by 129 nucleotides
BPHYT_RS10890: BPHYT_RS10890 - hypothetical protein, at 2,479,228 to 2,479,578
_RS10890
BPHYT_RS10895: BPHYT_RS10895 - transcriptional regulator, at 2,479,758 to 2,480,417
_RS10895
BPHYT_RS10900: BPHYT_RS10900 - histidine kinase, at 2,480,414 to 2,481,817
_RS10900
BPHYT_RS10905: BPHYT_RS10905 - membrane protein, at 2,481,947 to 2,482,942
_RS10905
Position (kb)
2480
2481
2482 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2479.645 kb on - strand at 2479.709 kb on - strand at 2479.757 kb on + strand at 2479.758 kb on - strand at 2479.758 kb on - strand at 2479.765 kb on + strand at 2479.765 kb on + strand at 2480.050 kb on - strand, within BPHYT_RS10895 at 2480.292 kb on + strand, within BPHYT_RS10895 at 2480.697 kb on + strand, within BPHYT_RS10900 at 2480.825 kb on + strand, within BPHYT_RS10900 at 2480.825 kb on + strand, within BPHYT_RS10900 at 2480.825 kb on + strand, within BPHYT_RS10900 at 2480.826 kb on - strand, within BPHYT_RS10900 at 2481.530 kb on + strand, within BPHYT_RS10900 at 2481.878 kb on - strand at 2482.694 kb on - strand, within BPHYT_RS10905
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,479,645 - +0.3 2,479,709 - -0.9 2,479,757 + +3.3 2,479,758 - -1.2 2,479,758 - +1.3 2,479,765 + -0.9 2,479,765 + +0.5 2,480,050 - BPHYT_RS10895 0.44 -0.3 2,480,292 + BPHYT_RS10895 0.81 -1.1 2,480,697 + BPHYT_RS10900 0.20 +0.1 2,480,825 + BPHYT_RS10900 0.29 +3.0 2,480,825 + BPHYT_RS10900 0.29 +1.3 2,480,825 + BPHYT_RS10900 0.29 +0.8 2,480,826 - BPHYT_RS10900 0.29 +1.3 2,481,530 + BPHYT_RS10900 0.79 +0.7 2,481,878 - -0.2 2,482,694 - BPHYT_RS10905 0.75 +0.8
Or see this region's nucleotide sequence