Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10420 and BPHYT_RS10425 are separated by 60 nucleotides BPHYT_RS10425 and BPHYT_RS10430 overlap by 4 nucleotides BPHYT_RS10430 and BPHYT_RS10435 are separated by 558 nucleotides
BPHYT_RS10420: BPHYT_RS10420 - phospholipase, at 2,368,189 to 2,369,742
_RS10420
BPHYT_RS10425: BPHYT_RS10425 - polysaccharide deacetylase, at 2,369,803 to 2,370,633
_RS10425
BPHYT_RS10430: BPHYT_RS10430 - glycosyl transferase family 1, at 2,370,630 to 2,371,745
_RS10430
BPHYT_RS10435: BPHYT_RS10435 - ThiJ/PfpI domain-containing protein, at 2,372,304 to 2,372,921
_RS10435
Position (kb)
2370
2371
2372 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2370.020 kb on + strand, within BPHYT_RS10425 at 2370.021 kb on - strand, within BPHYT_RS10425 at 2370.159 kb on + strand, within BPHYT_RS10425 at 2370.419 kb on + strand, within BPHYT_RS10425 at 2370.507 kb on + strand, within BPHYT_RS10425 at 2370.508 kb on - strand, within BPHYT_RS10425 at 2370.527 kb on + strand, within BPHYT_RS10425 at 2370.596 kb on + strand at 2370.597 kb on - strand at 2370.659 kb on - strand at 2370.674 kb on - strand at 2370.689 kb on - strand at 2371.277 kb on - strand, within BPHYT_RS10430 at 2371.372 kb on + strand, within BPHYT_RS10430 at 2371.603 kb on - strand, within BPHYT_RS10430 at 2371.868 kb on - strand at 2371.984 kb on - strand at 2372.158 kb on - strand at 2372.265 kb on + strand at 2372.365 kb on + strand at 2372.366 kb on - strand, within BPHYT_RS10435 at 2372.459 kb on - strand, within BPHYT_RS10435 at 2372.571 kb on - strand, within BPHYT_RS10435 at 2372.640 kb on + strand, within BPHYT_RS10435
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,370,020 + BPHYT_RS10425 0.26 +1.3 2,370,021 - BPHYT_RS10425 0.26 -1.9 2,370,159 + BPHYT_RS10425 0.43 -2.4 2,370,419 + BPHYT_RS10425 0.74 -0.3 2,370,507 + BPHYT_RS10425 0.85 -1.8 2,370,508 - BPHYT_RS10425 0.85 +0.5 2,370,527 + BPHYT_RS10425 0.87 +0.4 2,370,596 + +0.4 2,370,597 - -0.5 2,370,659 - +0.7 2,370,674 - +0.5 2,370,689 - -0.8 2,371,277 - BPHYT_RS10430 0.58 +0.8 2,371,372 + BPHYT_RS10430 0.66 +1.8 2,371,603 - BPHYT_RS10430 0.87 +0.4 2,371,868 - -1.5 2,371,984 - -0.3 2,372,158 - +0.7 2,372,265 + -0.3 2,372,365 + +0.3 2,372,366 - BPHYT_RS10435 0.10 -2.6 2,372,459 - BPHYT_RS10435 0.25 -0.3 2,372,571 - BPHYT_RS10435 0.43 +0.1 2,372,640 + BPHYT_RS10435 0.54 +0.9
Or see this region's nucleotide sequence