Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09795 and BPHYT_RS09800 are separated by 361 nucleotides BPHYT_RS09800 and BPHYT_RS09805 are separated by 251 nucleotides BPHYT_RS09805 and BPHYT_RS09810 are separated by 5 nucleotides BPHYT_RS09810 and BPHYT_RS09815 are separated by 153 nucleotides
BPHYT_RS09795: BPHYT_RS09795 - UTP--glucose-1-phosphate uridylyltransferase, at 2,225,931 to 2,226,848
_RS09795
BPHYT_RS09800: BPHYT_RS09800 - hypothetical protein, at 2,227,210 to 2,227,542
_RS09800
BPHYT_RS09805: BPHYT_RS09805 - hypothetical protein, at 2,227,794 to 2,228,591
_RS09805
BPHYT_RS09810: BPHYT_RS09810 - hypothetical protein, at 2,228,597 to 2,228,821
_RS09810
BPHYT_RS09815: BPHYT_RS09815 - malate synthase, at 2,228,975 to 2,231,149
_RS09815
Position (kb)
2227
2228
2229 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2226.930 kb on + strand at 2227.030 kb on + strand at 2227.227 kb on - strand at 2227.347 kb on + strand, within BPHYT_RS09800 at 2227.541 kb on + strand at 2227.541 kb on + strand at 2227.624 kb on + strand at 2227.668 kb on + strand at 2227.789 kb on + strand at 2227.963 kb on - strand, within BPHYT_RS09805 at 2228.082 kb on + strand, within BPHYT_RS09805 at 2228.091 kb on + strand, within BPHYT_RS09805 at 2228.092 kb on - strand, within BPHYT_RS09805 at 2228.157 kb on + strand, within BPHYT_RS09805 at 2228.232 kb on + strand, within BPHYT_RS09805 at 2228.236 kb on - strand, within BPHYT_RS09805 at 2228.237 kb on + strand, within BPHYT_RS09805 at 2228.238 kb on - strand, within BPHYT_RS09805 at 2228.495 kb on - strand, within BPHYT_RS09805 at 2228.548 kb on - strand at 2228.548 kb on - strand at 2228.691 kb on - strand, within BPHYT_RS09810 at 2228.762 kb on + strand, within BPHYT_RS09810 at 2228.762 kb on + strand, within BPHYT_RS09810 at 2228.802 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,226,930 + -0.7 2,227,030 + +0.8 2,227,227 - +1.1 2,227,347 + BPHYT_RS09800 0.41 -1.8 2,227,541 + +0.6 2,227,541 + +0.9 2,227,624 + -0.0 2,227,668 + +1.4 2,227,789 + -0.1 2,227,963 - BPHYT_RS09805 0.21 -1.8 2,228,082 + BPHYT_RS09805 0.36 +1.5 2,228,091 + BPHYT_RS09805 0.37 -0.2 2,228,092 - BPHYT_RS09805 0.37 +1.0 2,228,157 + BPHYT_RS09805 0.45 +3.1 2,228,232 + BPHYT_RS09805 0.55 +0.2 2,228,236 - BPHYT_RS09805 0.55 +2.8 2,228,237 + BPHYT_RS09805 0.56 -0.6 2,228,238 - BPHYT_RS09805 0.56 -3.2 2,228,495 - BPHYT_RS09805 0.88 +0.8 2,228,548 - +0.8 2,228,548 - -0.4 2,228,691 - BPHYT_RS09810 0.42 +0.5 2,228,762 + BPHYT_RS09810 0.73 -0.6 2,228,762 + BPHYT_RS09810 0.73 -0.6 2,228,802 - -0.7
Or see this region's nucleotide sequence