Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08545

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08540 and BPHYT_RS08545 are separated by 153 nucleotidesBPHYT_RS08545 and BPHYT_RS08550 are separated by 128 nucleotidesBPHYT_RS08550 and BPHYT_RS08555 are separated by 18 nucleotides BPHYT_RS08540: BPHYT_RS08540 - GntR family transcriptional regulator, at 1,953,472 to 1,954,197 _RS08540 BPHYT_RS08545: BPHYT_RS08545 - oxidoreductase, at 1,954,351 to 1,955,343 _RS08545 BPHYT_RS08550: BPHYT_RS08550 - signal peptidase, at 1,955,472 to 1,956,170 _RS08550 BPHYT_RS08555: BPHYT_RS08555 - ABC transporter permease, at 1,956,189 to 1,956,848 _RS08555 Position (kb) 1954 1955 1956Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1953.380 kb on + strandat 1954.906 kb on + strand, within BPHYT_RS08545at 1954.907 kb on - strand, within BPHYT_RS08545at 1955.342 kb on - strandat 1955.800 kb on - strand, within BPHYT_RS08550at 1956.102 kb on + strandat 1956.286 kb on + strand, within BPHYT_RS08555at 1956.287 kb on - strand, within BPHYT_RS08555at 1956.305 kb on - strand, within BPHYT_RS08555

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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1,953,380 + +1.7
1,954,906 + BPHYT_RS08545 0.56 -2.9
1,954,907 - BPHYT_RS08545 0.56 -2.4
1,955,342 - -0.3
1,955,800 - BPHYT_RS08550 0.47 -1.1
1,956,102 + -1.2
1,956,286 + BPHYT_RS08555 0.15 +1.8
1,956,287 - BPHYT_RS08555 0.15 -0.4
1,956,305 - BPHYT_RS08555 0.18 +0.4

Or see this region's nucleotide sequence