Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08510 and BPHYT_RS08515 overlap by 4 nucleotides BPHYT_RS08515 and BPHYT_RS08520 are separated by 324 nucleotides BPHYT_RS08520 and BPHYT_RS08525 are separated by 440 nucleotides
BPHYT_RS08510: BPHYT_RS08510 - acyl-CoA dehydrogenase, at 1,947,585 to 1,948,871
_RS08510
BPHYT_RS08515: BPHYT_RS08515 - acyl carrier protein, at 1,948,868 to 1,949,119
_RS08515
BPHYT_RS08520: BPHYT_RS08520 - GNAT family acetyltransferase, at 1,949,444 to 1,949,977
_RS08520
BPHYT_RS08525: BPHYT_RS08525 - SAM-dependent methyltransferase, at 1,950,418 to 1,951,251
_RS08525
Position (kb) 1949
1950 Strain fitness (log2 ratio) -2
-1
0
1 at 1948.534 kb on + strand, within BPHYT_RS08510 at 1948.534 kb on + strand, within BPHYT_RS08510 at 1948.534 kb on + strand, within BPHYT_RS08510 at 1948.535 kb on - strand, within BPHYT_RS08510 at 1949.132 kb on + strand at 1949.225 kb on + strand at 1949.350 kb on + strand at 1949.351 kb on - strand at 1950.162 kb on - strand at 1950.367 kb on + strand at 1950.367 kb on + strand at 1950.368 kb on - strand at 1950.368 kb on - strand at 1950.399 kb on - strand at 1950.746 kb on - strand, within BPHYT_RS08525
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 1,948,534 + BPHYT_RS08510 0.74 +0.4 1,948,534 + BPHYT_RS08510 0.74 +0.3 1,948,534 + BPHYT_RS08510 0.74 -1.0 1,948,535 - BPHYT_RS08510 0.74 +0.7 1,949,132 + +0.9 1,949,225 + +0.9 1,949,350 + +1.3 1,949,351 - -0.3 1,950,162 - +1.1 1,950,367 + +0.1 1,950,367 + -0.3 1,950,368 - -2.0 1,950,368 - +1.2 1,950,399 - +0.9 1,950,746 - BPHYT_RS08525 0.39 -2.0
Or see this region's nucleotide sequence