Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08200

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08195 and BPHYT_RS08200 are separated by 9 nucleotidesBPHYT_RS08200 and BPHYT_RS08210 are separated by 450 nucleotides BPHYT_RS08195: BPHYT_RS08195 - type II secretion system protein, at 1,873,964 to 1,874,974 _RS08195 BPHYT_RS08200: BPHYT_RS08200 - pilus assembly protein, at 1,874,984 to 1,875,838 _RS08200 BPHYT_RS08210: BPHYT_RS08210 - membrane protein, at 1,876,289 to 1,878,079 _RS08210 Position (kb) 1874 1875 1876Strain fitness (log2 ratio) -1 0 1at 1874.321 kb on - strand, within BPHYT_RS08195at 1875.173 kb on + strand, within BPHYT_RS08200at 1875.212 kb on + strand, within BPHYT_RS08200at 1875.212 kb on + strand, within BPHYT_RS08200at 1875.212 kb on + strand, within BPHYT_RS08200at 1876.163 kb on - strandat 1876.613 kb on - strand, within BPHYT_RS08210at 1876.650 kb on - strand, within BPHYT_RS08210at 1876.713 kb on - strand, within BPHYT_RS08210at 1876.754 kb on - strand, within BPHYT_RS08210at 1876.814 kb on - strand, within BPHYT_RS08210

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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1,874,321 - BPHYT_RS08195 0.35 +0.4
1,875,173 + BPHYT_RS08200 0.22 -1.1
1,875,212 + BPHYT_RS08200 0.27 +1.4
1,875,212 + BPHYT_RS08200 0.27 +0.7
1,875,212 + BPHYT_RS08200 0.27 +1.6
1,876,163 - -0.8
1,876,613 - BPHYT_RS08210 0.18 -0.8
1,876,650 - BPHYT_RS08210 0.20 -0.3
1,876,713 - BPHYT_RS08210 0.24 +0.7
1,876,754 - BPHYT_RS08210 0.26 +0.1
1,876,814 - BPHYT_RS08210 0.29 +0.6

Or see this region's nucleotide sequence