Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08155 and BPHYT_RS08160 are separated by 140 nucleotides BPHYT_RS08160 and BPHYT_RS08165 overlap by 4 nucleotides BPHYT_RS08165 and BPHYT_RS08170 are separated by 100 nucleotides
BPHYT_RS08155: BPHYT_RS08155 - pilus biosynthesis protein, at 1,866,339 to 1,866,518
_RS08155
BPHYT_RS08160: BPHYT_RS08160 - membrane protein, at 1,866,659 to 1,867,159
_RS08160
BPHYT_RS08165: BPHYT_RS08165 - pilus assembly protein TadE, at 1,867,156 to 1,867,653
_RS08165
BPHYT_RS08170: BPHYT_RS08170 - pilus assembly protein CpaB, at 1,867,754 to 1,868,704
_RS08170
Position (kb) 1867
1868 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1866.390 kb on - strand, within BPHYT_RS08155 at 1866.517 kb on - strand at 1866.530 kb on + strand at 1866.583 kb on + strand at 1866.684 kb on + strand at 1866.736 kb on + strand, within BPHYT_RS08160 at 1866.736 kb on + strand, within BPHYT_RS08160 at 1866.736 kb on + strand, within BPHYT_RS08160 at 1866.737 kb on - strand, within BPHYT_RS08160 at 1866.737 kb on - strand, within BPHYT_RS08160 at 1866.737 kb on - strand, within BPHYT_RS08160 at 1866.737 kb on - strand, within BPHYT_RS08160 at 1867.010 kb on - strand, within BPHYT_RS08160 at 1867.010 kb on - strand, within BPHYT_RS08160 at 1867.540 kb on + strand, within BPHYT_RS08165 at 1868.606 kb on - strand, within BPHYT_RS08170 at 1868.647 kb on + strand at 1868.648 kb on - strand at 1868.648 kb on - strand at 1868.648 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 1,866,390 - BPHYT_RS08155 0.28 -1.7 1,866,517 - -1.0 1,866,530 + +0.1 1,866,583 + -1.0 1,866,684 + -0.4 1,866,736 + BPHYT_RS08160 0.15 -2.3 1,866,736 + BPHYT_RS08160 0.15 -1.8 1,866,736 + BPHYT_RS08160 0.15 +0.0 1,866,737 - BPHYT_RS08160 0.16 -1.0 1,866,737 - BPHYT_RS08160 0.16 -2.5 1,866,737 - BPHYT_RS08160 0.16 -1.5 1,866,737 - BPHYT_RS08160 0.16 -1.4 1,867,010 - BPHYT_RS08160 0.70 +0.8 1,867,010 - BPHYT_RS08160 0.70 +0.2 1,867,540 + BPHYT_RS08165 0.77 -3.2 1,868,606 - BPHYT_RS08170 0.90 -0.5 1,868,647 + -0.5 1,868,648 - +0.2 1,868,648 - -0.6 1,868,648 - +1.7
Or see this region's nucleotide sequence