Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS08165

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS08155 and BPHYT_RS08160 are separated by 140 nucleotidesBPHYT_RS08160 and BPHYT_RS08165 overlap by 4 nucleotidesBPHYT_RS08165 and BPHYT_RS08170 are separated by 100 nucleotides BPHYT_RS08155: BPHYT_RS08155 - pilus biosynthesis protein, at 1,866,339 to 1,866,518 _RS08155 BPHYT_RS08160: BPHYT_RS08160 - membrane protein, at 1,866,659 to 1,867,159 _RS08160 BPHYT_RS08165: BPHYT_RS08165 - pilus assembly protein TadE, at 1,867,156 to 1,867,653 _RS08165 BPHYT_RS08170: BPHYT_RS08170 - pilus assembly protein CpaB, at 1,867,754 to 1,868,704 _RS08170 Position (kb) 1867 1868Strain fitness (log2 ratio) -3 -2 -1 0 1at 1866.390 kb on - strand, within BPHYT_RS08155at 1866.517 kb on - strandat 1866.530 kb on + strandat 1866.583 kb on + strandat 1866.684 kb on + strandat 1866.736 kb on + strand, within BPHYT_RS08160at 1866.736 kb on + strand, within BPHYT_RS08160at 1866.736 kb on + strand, within BPHYT_RS08160at 1866.737 kb on - strand, within BPHYT_RS08160at 1866.737 kb on - strand, within BPHYT_RS08160at 1866.737 kb on - strand, within BPHYT_RS08160at 1866.737 kb on - strand, within BPHYT_RS08160at 1867.010 kb on - strand, within BPHYT_RS08160at 1867.010 kb on - strand, within BPHYT_RS08160at 1867.540 kb on + strand, within BPHYT_RS08165at 1868.606 kb on - strand, within BPHYT_RS08170at 1868.647 kb on + strandat 1868.648 kb on - strandat 1868.648 kb on - strandat 1868.648 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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1,866,390 - BPHYT_RS08155 0.28 -1.7
1,866,517 - -1.0
1,866,530 + +0.1
1,866,583 + -1.0
1,866,684 + -0.4
1,866,736 + BPHYT_RS08160 0.15 -2.3
1,866,736 + BPHYT_RS08160 0.15 -1.8
1,866,736 + BPHYT_RS08160 0.15 +0.0
1,866,737 - BPHYT_RS08160 0.16 -1.0
1,866,737 - BPHYT_RS08160 0.16 -2.5
1,866,737 - BPHYT_RS08160 0.16 -1.5
1,866,737 - BPHYT_RS08160 0.16 -1.4
1,867,010 - BPHYT_RS08160 0.70 +0.8
1,867,010 - BPHYT_RS08160 0.70 +0.2
1,867,540 + BPHYT_RS08165 0.77 -3.2
1,868,606 - BPHYT_RS08170 0.90 -0.5
1,868,647 + -0.5
1,868,648 - +0.2
1,868,648 - -0.6
1,868,648 - +1.7

Or see this region's nucleotide sequence