Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS06830

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS06815 and BPHYT_RS06820 are separated by 17 nucleotidesBPHYT_RS06820 and BPHYT_RS06825 are separated by 68 nucleotidesBPHYT_RS06825 and BPHYT_RS06830 are separated by 314 nucleotidesBPHYT_RS06830 and BPHYT_RS06835 are separated by 189 nucleotidesBPHYT_RS06835 and BPHYT_RS06840 are separated by 314 nucleotides BPHYT_RS06815: BPHYT_RS06815 - 16S ribosomal RNA, at 1,541,569 to 1,543,163 _RS06815 BPHYT_RS06820: BPHYT_RS06820 - tRNA-Ile, at 1,543,181 to 1,543,257 _RS06820 BPHYT_RS06825: BPHYT_RS06825 - tRNA-Ala, at 1,543,326 to 1,543,401 _RS06825 BPHYT_RS06830: BPHYT_RS06830 - 23S ribosomal RNA, at 1,543,716 to 1,546,618 _RS06830 BPHYT_RS06835: BPHYT_RS06835 - 5S ribosomal RNA, at 1,546,808 to 1,546,921 _RS06835 BPHYT_RS06840: BPHYT_RS06840 - hypothetical protein, at 1,547,236 to 1,548,075 _RS06840 Position (kb) 1543 1544 1545 1546 1547Strain fitness (log2 ratio) -1 0 1at 1547.438 kb on + strand, within BPHYT_RS06840at 1547.439 kb on - strand, within BPHYT_RS06840at 1547.568 kb on - strand, within BPHYT_RS06840at 1547.568 kb on - strand, within BPHYT_RS06840

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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1,547,438 + BPHYT_RS06840 0.24 +1.0
1,547,439 - BPHYT_RS06840 0.24 +0.1
1,547,568 - BPHYT_RS06840 0.40 +0.9
1,547,568 - BPHYT_RS06840 0.40 +0.3

Or see this region's nucleotide sequence