Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05575 and BPHYT_RS05580 are separated by 103 nucleotides BPHYT_RS05580 and BPHYT_RS05585 are separated by 181 nucleotides BPHYT_RS05585 and BPHYT_RS05590 are separated by 2 nucleotides BPHYT_RS05590 and BPHYT_RS05595 are separated by 155 nucleotides
BPHYT_RS05575: BPHYT_RS05575 - Fis family transcriptional regulator, at 1,279,406 to 1,280,806
_RS05575
BPHYT_RS05580: BPHYT_RS05580 - hypothetical protein, at 1,280,910 to 1,281,179
_RS05580
BPHYT_RS05585: BPHYT_RS05585 - hypothetical protein, at 1,281,361 to 1,281,759
_RS05585
BPHYT_RS05590: BPHYT_RS05590 - trehalose-6-phosphate synthase, at 1,281,762 to 1,283,198
_RS05590
BPHYT_RS05595: BPHYT_RS05595 - multidrug MFS transporter, at 1,283,354 to 1,284,727
_RS05595
Position (kb) 1281
1282
1283
1284 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1280.829 kb on + strand at 1280.829 kb on + strand at 1280.830 kb on - strand at 1280.903 kb on + strand at 1280.909 kb on + strand at 1280.909 kb on + strand at 1280.910 kb on - strand at 1280.920 kb on - strand at 1280.920 kb on - strand at 1280.920 kb on - strand at 1280.920 kb on - strand at 1280.920 kb on - strand at 1281.030 kb on - strand, within BPHYT_RS05580 at 1281.030 kb on - strand, within BPHYT_RS05580 at 1281.170 kb on - strand at 1281.170 kb on - strand at 1281.231 kb on - strand at 1281.237 kb on + strand at 1281.238 kb on - strand at 1281.238 kb on - strand at 1281.269 kb on - strand at 1281.269 kb on - strand at 1281.269 kb on - strand at 1281.290 kb on + strand at 1281.291 kb on - strand at 1281.515 kb on - strand, within BPHYT_RS05585 at 1281.722 kb on - strand at 1281.722 kb on - strand at 1281.925 kb on + strand, within BPHYT_RS05590 at 1281.949 kb on - strand, within BPHYT_RS05590 at 1281.979 kb on - strand, within BPHYT_RS05590 at 1281.987 kb on + strand, within BPHYT_RS05590 at 1281.987 kb on + strand at 1281.987 kb on + strand, within BPHYT_RS05590 at 1281.988 kb on - strand, within BPHYT_RS05590 at 1281.991 kb on - strand, within BPHYT_RS05590 at 1281.991 kb on - strand, within BPHYT_RS05590 at 1282.077 kb on + strand, within BPHYT_RS05590 at 1282.156 kb on - strand, within BPHYT_RS05590 at 1282.299 kb on + strand, within BPHYT_RS05590 at 1282.299 kb on + strand, within BPHYT_RS05590 at 1282.300 kb on - strand, within BPHYT_RS05590 at 1282.404 kb on + strand, within BPHYT_RS05590 at 1282.404 kb on + strand, within BPHYT_RS05590 at 1282.405 kb on - strand, within BPHYT_RS05590 at 1282.406 kb on + strand, within BPHYT_RS05590 at 1282.407 kb on - strand, within BPHYT_RS05590 at 1282.447 kb on - strand, within BPHYT_RS05590 at 1282.447 kb on - strand, within BPHYT_RS05590 at 1282.875 kb on + strand, within BPHYT_RS05590 at 1283.099 kb on + strand at 1283.100 kb on - strand at 1283.437 kb on - strand at 1283.831 kb on + strand, within BPHYT_RS05595 at 1284.126 kb on + strand, within BPHYT_RS05595
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 1,280,829 + -0.3 1,280,829 + -0.5 1,280,830 - +0.1 1,280,903 + -0.6 1,280,909 + +0.1 1,280,909 + +0.5 1,280,910 - +0.8 1,280,920 - +0.1 1,280,920 - -2.0 1,280,920 - -0.3 1,280,920 - +2.0 1,280,920 - +0.4 1,281,030 - BPHYT_RS05580 0.44 -0.1 1,281,030 - BPHYT_RS05580 0.44 -0.2 1,281,170 - +0.8 1,281,170 - -2.1 1,281,231 - +0.8 1,281,237 + +0.1 1,281,238 - +0.7 1,281,238 - +1.4 1,281,269 - +0.4 1,281,269 - +0.6 1,281,269 - +0.8 1,281,290 + +0.6 1,281,291 - +1.1 1,281,515 - BPHYT_RS05585 0.39 +0.6 1,281,722 - +0.2 1,281,722 - +0.6 1,281,925 + BPHYT_RS05590 0.11 +0.5 1,281,949 - BPHYT_RS05590 0.13 +1.0 1,281,979 - BPHYT_RS05590 0.15 -0.5 1,281,987 + BPHYT_RS05590 0.16 +1.1 1,281,987 + +2.2 1,281,987 + BPHYT_RS05590 0.16 +0.7 1,281,988 - BPHYT_RS05590 0.16 +0.3 1,281,991 - BPHYT_RS05590 0.16 +0.5 1,281,991 - BPHYT_RS05590 0.16 +0.8 1,282,077 + BPHYT_RS05590 0.22 +0.5 1,282,156 - BPHYT_RS05590 0.27 +3.1 1,282,299 + BPHYT_RS05590 0.37 +0.7 1,282,299 + BPHYT_RS05590 0.37 +0.7 1,282,300 - BPHYT_RS05590 0.37 +0.7 1,282,404 + BPHYT_RS05590 0.45 +1.1 1,282,404 + BPHYT_RS05590 0.45 +0.8 1,282,405 - BPHYT_RS05590 0.45 +1.2 1,282,406 + BPHYT_RS05590 0.45 +0.6 1,282,407 - BPHYT_RS05590 0.45 +0.8 1,282,447 - BPHYT_RS05590 0.48 +0.7 1,282,447 - BPHYT_RS05590 0.48 +0.5 1,282,875 + BPHYT_RS05590 0.77 +0.4 1,283,099 + +0.5 1,283,100 - +0.7 1,283,437 - -1.5 1,283,831 + BPHYT_RS05595 0.35 -3.1 1,284,126 + BPHYT_RS05595 0.56 -0.1
Or see this region's nucleotide sequence