Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS05395

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS05390 and BPHYT_RS05395 are separated by 39 nucleotidesBPHYT_RS05395 and BPHYT_RS05400 are separated by 344 nucleotidesBPHYT_RS05400 and BPHYT_RS05405 are separated by 33 nucleotides BPHYT_RS05390: BPHYT_RS05390 - isochorismatase, at 1,239,752 to 1,240,399 _RS05390 BPHYT_RS05395: BPHYT_RS05395 - hypothetical protein, at 1,240,439 to 1,240,627 _RS05395 BPHYT_RS05400: BPHYT_RS05400 - hypothetical protein, at 1,240,972 to 1,241,418 _RS05400 BPHYT_RS05405: BPHYT_RS05405 - GCN5 family acetyltransferase, at 1,241,452 to 1,241,955 _RS05405 Position (kb) 1240 1241Strain fitness (log2 ratio) -1 0 1at 1239.771 kb on - strandat 1240.065 kb on + strand, within BPHYT_RS05390at 1240.249 kb on + strand, within BPHYT_RS05390at 1240.250 kb on - strand, within BPHYT_RS05390at 1240.367 kb on - strandat 1240.441 kb on - strandat 1240.517 kb on - strand, within BPHYT_RS05395at 1241.499 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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1,239,771 - -0.6
1,240,065 + BPHYT_RS05390 0.48 +0.0
1,240,249 + BPHYT_RS05390 0.77 +0.1
1,240,250 - BPHYT_RS05390 0.77 -1.4
1,240,367 - +0.4
1,240,441 - +0.5
1,240,517 - BPHYT_RS05395 0.41 +1.1
1,241,499 - +0.2

Or see this region's nucleotide sequence