Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05000 and BPHYT_RS05005 are separated by 116 nucleotides BPHYT_RS05005 and BPHYT_RS05010 are separated by 161 nucleotides BPHYT_RS05010 and BPHYT_RS05015 overlap by 23 nucleotides BPHYT_RS05015 and BPHYT_RS05020 are separated by 180 nucleotides
BPHYT_RS05000: BPHYT_RS05000 - nucleotide pyrophosphatase, at 1,151,332 to 1,153,005
_RS05000
BPHYT_RS05005: BPHYT_RS05005 - hypothetical protein, at 1,153,122 to 1,153,475
_RS05005
BPHYT_RS05010: BPHYT_RS05010 - RpiR family transcriptional regulator, at 1,153,637 to 1,155,553
_RS05010
BPHYT_RS05015: BPHYT_RS05015 - 6-phosphogluconolactonase, at 1,155,531 to 1,156,235
_RS05015
BPHYT_RS05020: BPHYT_RS05020 - glucose-6-phosphate 1-dehydrogenase, at 1,156,416 to 1,157,879
_RS05020
Position (kb)
1153
1154
1155
1156 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1152.661 kb on + strand, within BPHYT_RS05000 at 1152.661 kb on + strand, within BPHYT_RS05000 at 1153.069 kb on + strand at 1153.122 kb on - strand at 1153.248 kb on - strand, within BPHYT_RS05005 at 1153.354 kb on - strand, within BPHYT_RS05005 at 1153.530 kb on - strand at 1153.530 kb on - strand at 1153.786 kb on + strand at 1154.062 kb on + strand, within BPHYT_RS05010 at 1154.089 kb on - strand, within BPHYT_RS05010 at 1154.617 kb on - strand, within BPHYT_RS05010 at 1154.617 kb on - strand, within BPHYT_RS05010 at 1154.731 kb on - strand, within BPHYT_RS05010 at 1154.731 kb on - strand, within BPHYT_RS05010 at 1155.124 kb on + strand, within BPHYT_RS05010 at 1155.367 kb on - strand at 1155.533 kb on - strand at 1156.290 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 1,152,661 + BPHYT_RS05000 0.79 -0.6 1,152,661 + BPHYT_RS05000 0.79 -2.9 1,153,069 + -2.1 1,153,122 - -0.2 1,153,248 - BPHYT_RS05005 0.36 -1.2 1,153,354 - BPHYT_RS05005 0.66 -0.3 1,153,530 - -0.7 1,153,530 - -0.2 1,153,786 + +1.2 1,154,062 + BPHYT_RS05010 0.22 +0.9 1,154,089 - BPHYT_RS05010 0.24 +0.2 1,154,617 - BPHYT_RS05010 0.51 -0.9 1,154,617 - BPHYT_RS05010 0.51 +1.4 1,154,731 - BPHYT_RS05010 0.57 +0.0 1,154,731 - BPHYT_RS05010 0.57 +2.1 1,155,124 + BPHYT_RS05010 0.78 +1.4 1,155,367 - +1.9 1,155,533 - +0.1 1,156,290 - -1.1
Or see this region's nucleotide sequence