Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS04990

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS04980 and BPHYT_RS04985 overlap by 16 nucleotidesBPHYT_RS04985 and BPHYT_RS04990 are separated by 39 nucleotidesBPHYT_RS04990 and BPHYT_RS04995 are separated by 703 nucleotides BPHYT_RS04980: BPHYT_RS04980 - lysine decarboxylase, at 1,145,961 to 1,148,246 _RS04980 BPHYT_RS04985: BPHYT_RS04985 - hypothetical protein, at 1,148,231 to 1,148,992 _RS04985 BPHYT_RS04990: BPHYT_RS04990 - hypothetical protein, at 1,149,032 to 1,149,958 _RS04990 BPHYT_RS04995: BPHYT_RS04995 - hypothetical protein, at 1,150,662 to 1,151,114 _RS04995 Position (kb) 1149 1150Strain fitness (log2 ratio) -2 -1 0 1at 1148.642 kb on + strand, within BPHYT_RS04985at 1148.987 kb on - strandat 1148.987 kb on - strandat 1149.210 kb on + strand, within BPHYT_RS04990at 1149.771 kb on - strand, within BPHYT_RS04990at 1149.777 kb on - strand, within BPHYT_RS04990at 1150.321 kb on + strandat 1150.921 kb on + strand, within BPHYT_RS04995

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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1,148,642 + BPHYT_RS04985 0.54 +0.9
1,148,987 - +0.2
1,148,987 - -1.9
1,149,210 + BPHYT_RS04990 0.19 -1.1
1,149,771 - BPHYT_RS04990 0.80 +1.7
1,149,777 - BPHYT_RS04990 0.80 +0.6
1,150,321 + -0.5
1,150,921 + BPHYT_RS04995 0.57 +0.1

Or see this region's nucleotide sequence