Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03515 and BPHYT_RS03520 are separated by 216 nucleotides BPHYT_RS03520 and BPHYT_RS03525 are separated by 73 nucleotides BPHYT_RS03525 and BPHYT_RS03530 are separated by 19 nucleotides BPHYT_RS03530 and BPHYT_RS03535 are separated by 189 nucleotides
BPHYT_RS03515: BPHYT_RS03515 - D-lactate dehydrogenase, at 792,972 to 794,387
_RS03515
BPHYT_RS03520: BPHYT_RS03520 - ATP--cobalamin adenosyltransferase, at 794,604 to 795,158
_RS03520
BPHYT_RS03525: BPHYT_RS03525 - XRE family transcriptional regulator, at 795,232 to 795,564
_RS03525
BPHYT_RS03530: BPHYT_RS03530 - hypothetical protein, at 795,584 to 795,931
_RS03530
BPHYT_RS03535: BPHYT_RS03535 - phospho-2-dehydro-3-deoxyheptonate aldolase, at 796,121 to 797,194
_RS03535
Position (kb)
794
795
796 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 793.626 kb on + strand, within BPHYT_RS03515 at 793.720 kb on + strand, within BPHYT_RS03515 at 793.793 kb on - strand, within BPHYT_RS03515 at 793.897 kb on - strand, within BPHYT_RS03515 at 793.990 kb on - strand, within BPHYT_RS03515 at 794.168 kb on - strand, within BPHYT_RS03515 at 794.332 kb on - strand at 794.401 kb on + strand at 794.401 kb on + strand at 794.485 kb on + strand at 794.486 kb on - strand at 794.487 kb on + strand at 794.590 kb on - strand at 795.054 kb on + strand, within BPHYT_RS03520 at 795.054 kb on + strand, within BPHYT_RS03520 at 795.156 kb on + strand at 795.175 kb on - strand at 795.579 kb on + strand at 795.696 kb on + strand, within BPHYT_RS03530 at 795.696 kb on + strand, within BPHYT_RS03530 at 795.696 kb on + strand, within BPHYT_RS03530 at 795.697 kb on - strand, within BPHYT_RS03530 at 795.723 kb on + strand, within BPHYT_RS03530 at 795.723 kb on + strand, within BPHYT_RS03530 at 795.723 kb on + strand, within BPHYT_RS03530 at 795.724 kb on - strand, within BPHYT_RS03530 at 795.724 kb on - strand, within BPHYT_RS03530 at 795.724 kb on - strand, within BPHYT_RS03530 at 795.724 kb on - strand, within BPHYT_RS03530 at 795.724 kb on - strand, within BPHYT_RS03530 at 795.724 kb on - strand, within BPHYT_RS03530 at 795.902 kb on + strand at 796.008 kb on + strand at 796.025 kb on + strand at 796.026 kb on - strand at 796.092 kb on + strand at 796.093 kb on - strand at 796.110 kb on + strand at 796.123 kb on - strand at 796.123 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 793,626 + BPHYT_RS03515 0.46 -0.3 793,720 + BPHYT_RS03515 0.53 -0.1 793,793 - BPHYT_RS03515 0.58 -0.5 793,897 - BPHYT_RS03515 0.65 -1.1 793,990 - BPHYT_RS03515 0.72 -2.2 794,168 - BPHYT_RS03515 0.84 -0.8 794,332 - +2.1 794,401 + +0.8 794,401 + -1.9 794,485 + +0.2 794,486 - +0.1 794,487 + -1.8 794,590 - -0.3 795,054 + BPHYT_RS03520 0.81 +0.3 795,054 + BPHYT_RS03520 0.81 +1.8 795,156 + +0.5 795,175 - -0.0 795,579 + -0.1 795,696 + BPHYT_RS03530 0.32 +1.7 795,696 + BPHYT_RS03530 0.32 -1.4 795,696 + BPHYT_RS03530 0.32 +0.4 795,697 - BPHYT_RS03530 0.32 -2.5 795,723 + BPHYT_RS03530 0.40 -1.2 795,723 + BPHYT_RS03530 0.40 -0.5 795,723 + BPHYT_RS03530 0.40 -2.2 795,724 - BPHYT_RS03530 0.40 -0.3 795,724 - BPHYT_RS03530 0.40 +2.1 795,724 - BPHYT_RS03530 0.40 -0.5 795,724 - BPHYT_RS03530 0.40 +0.2 795,724 - BPHYT_RS03530 0.40 +0.7 795,724 - BPHYT_RS03530 0.40 -0.9 795,902 + +0.7 796,008 + +0.9 796,025 + -0.6 796,026 - -0.5 796,092 + +1.2 796,093 - -0.9 796,110 + -0.3 796,123 - -1.2 796,123 - +0.3
Or see this region's nucleotide sequence