Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03250 and BPHYT_RS03255 are separated by 19 nucleotides BPHYT_RS03255 and BPHYT_RS03260 are separated by 160 nucleotides BPHYT_RS03260 and BPHYT_RS03265 are separated by 195 nucleotides BPHYT_RS03265 and BPHYT_RS03270 are separated by 68 nucleotides
BPHYT_RS03250: BPHYT_RS03250 - diguanylate cyclase, at 730,026 to 733,136
_RS03250
BPHYT_RS03255: BPHYT_RS03255 - glutathione S-transferase, at 733,156 to 733,770
_RS03255
BPHYT_RS03260: BPHYT_RS03260 - glycine cleavage system regulatory protein, at 733,931 to 734,860
_RS03260
BPHYT_RS03265: BPHYT_RS03265 - hypothetical protein, at 735,056 to 735,394
_RS03265
BPHYT_RS03270: BPHYT_RS03270 - acyl-CoA hydrolase, at 735,463 to 735,858
_RS03270
Position (kb) 733
734
735 Strain fitness (log2 ratio) -2
-1
0
1
2 at 733.056 kb on + strand at 733.057 kb on - strand at 733.057 kb on - strand at 733.057 kb on - strand at 733.111 kb on + strand at 733.112 kb on - strand at 733.566 kb on - strand, within BPHYT_RS03255 at 733.661 kb on + strand, within BPHYT_RS03255 at 733.662 kb on - strand, within BPHYT_RS03255 at 733.709 kb on - strand at 733.791 kb on + strand at 733.792 kb on - strand at 733.869 kb on + strand at 733.869 kb on + strand at 733.902 kb on + strand at 733.903 kb on - strand at 734.291 kb on + strand, within BPHYT_RS03260 at 734.291 kb on + strand, within BPHYT_RS03260 at 734.729 kb on + strand, within BPHYT_RS03260 at 734.813 kb on + strand at 734.813 kb on + strand at 734.813 kb on + strand at 734.813 kb on + strand at 734.814 kb on - strand at 734.814 kb on - strand at 734.866 kb on - strand at 734.878 kb on - strand at 734.908 kb on - strand at 734.908 kb on - strand at 735.105 kb on + strand, within BPHYT_RS03265 at 735.105 kb on + strand, within BPHYT_RS03265 at 735.106 kb on - strand, within BPHYT_RS03265 at 735.403 kb on + strand at 735.403 kb on + strand at 735.404 kb on - strand at 735.404 kb on - strand at 735.404 kb on - strand at 735.404 kb on - strand at 735.515 kb on + strand, within BPHYT_RS03270 at 735.516 kb on - strand, within BPHYT_RS03270 at 735.516 kb on - strand, within BPHYT_RS03270 at 735.576 kb on - strand, within BPHYT_RS03270 at 735.604 kb on - strand, within BPHYT_RS03270 at 735.629 kb on + strand, within BPHYT_RS03270 at 735.630 kb on - strand, within BPHYT_RS03270 at 735.849 kb on + strand at 735.849 kb on + strand at 735.849 kb on + strand at 735.850 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 733,056 + +0.6 733,057 - -0.5 733,057 - -1.8 733,057 - +0.0 733,111 + +0.0 733,112 - -0.0 733,566 - BPHYT_RS03255 0.67 +0.4 733,661 + BPHYT_RS03255 0.82 +1.0 733,662 - BPHYT_RS03255 0.82 -0.6 733,709 - -0.6 733,791 + +0.5 733,792 - -0.8 733,869 + -0.0 733,869 + +0.2 733,902 + -0.9 733,903 - -1.8 734,291 + BPHYT_RS03260 0.39 -1.6 734,291 + BPHYT_RS03260 0.39 -1.4 734,729 + BPHYT_RS03260 0.86 -1.0 734,813 + -0.1 734,813 + +0.8 734,813 + -0.7 734,813 + +0.9 734,814 - +1.7 734,814 - +2.1 734,866 - +0.5 734,878 - -0.2 734,908 - -0.1 734,908 - +1.0 735,105 + BPHYT_RS03265 0.14 -1.6 735,105 + BPHYT_RS03265 0.14 -0.9 735,106 - BPHYT_RS03265 0.15 -1.2 735,403 + +0.4 735,403 + -0.1 735,404 - -1.4 735,404 - -0.3 735,404 - -0.9 735,404 - -0.8 735,515 + BPHYT_RS03270 0.13 +1.3 735,516 - BPHYT_RS03270 0.13 -0.2 735,516 - BPHYT_RS03270 0.13 -0.7 735,576 - BPHYT_RS03270 0.29 +0.5 735,604 - BPHYT_RS03270 0.36 -0.8 735,629 + BPHYT_RS03270 0.42 -0.0 735,630 - BPHYT_RS03270 0.42 +1.2 735,849 + -1.4 735,849 + -0.1 735,849 + -0.1 735,850 - -0.3
Or see this region's nucleotide sequence