Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03125 and BPHYT_RS03130 are separated by 74 nucleotides BPHYT_RS03130 and BPHYT_RS03135 are separated by 74 nucleotides BPHYT_RS03135 and BPHYT_RS03140 overlap by 4 nucleotides BPHYT_RS03140 and BPHYT_RS03145 are separated by 23 nucleotides
BPHYT_RS03125: BPHYT_RS03125 - D-aminoacylase, at 699,193 to 700,674
_RS03125
BPHYT_RS03130: BPHYT_RS03130 - hypothetical protein, at 700,749 to 701,135
_RS03130
BPHYT_RS03135: BPHYT_RS03135 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 701,210 to 702,034
_RS03135
BPHYT_RS03140: BPHYT_RS03140 - 5'-nucleotidase, at 702,031 to 702,933
_RS03140
BPHYT_RS03145: BPHYT_RS03145 - threonine dehydratase, at 702,957 to 704,480
_RS03145
Position (kb) 701
702
703 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 700.225 kb on + strand, within BPHYT_RS03125 at 700.360 kb on + strand, within BPHYT_RS03125 at 700.360 kb on + strand, within BPHYT_RS03125 at 700.360 kb on + strand, within BPHYT_RS03125 at 700.360 kb on + strand, within BPHYT_RS03125 at 700.433 kb on - strand, within BPHYT_RS03125 at 700.433 kb on - strand, within BPHYT_RS03125 at 700.461 kb on + strand, within BPHYT_RS03125 at 700.735 kb on + strand at 700.736 kb on - strand at 700.759 kb on - strand at 700.759 kb on - strand at 700.796 kb on + strand, within BPHYT_RS03130 at 701.031 kb on + strand, within BPHYT_RS03130 at 701.115 kb on + strand at 701.116 kb on - strand at 701.117 kb on + strand at 701.117 kb on + strand at 701.118 kb on - strand at 701.212 kb on - strand at 701.212 kb on - strand at 701.253 kb on + strand at 701.254 kb on - strand at 701.297 kb on - strand, within BPHYT_RS03135 at 701.298 kb on + strand, within BPHYT_RS03135 at 701.307 kb on + strand, within BPHYT_RS03135 at 701.307 kb on + strand, within BPHYT_RS03135 at 701.364 kb on - strand, within BPHYT_RS03135 at 701.580 kb on + strand, within BPHYT_RS03135 at 701.581 kb on - strand, within BPHYT_RS03135 at 701.581 kb on - strand, within BPHYT_RS03135 at 701.581 kb on - strand, within BPHYT_RS03135 at 701.581 kb on - strand, within BPHYT_RS03135 at 701.772 kb on + strand, within BPHYT_RS03135 at 701.880 kb on + strand, within BPHYT_RS03135 at 701.880 kb on + strand, within BPHYT_RS03135 at 701.881 kb on - strand, within BPHYT_RS03135 at 701.881 kb on - strand, within BPHYT_RS03135 at 701.982 kb on + strand at 701.983 kb on - strand at 702.265 kb on - strand, within BPHYT_RS03140 at 702.270 kb on + strand, within BPHYT_RS03140 at 702.270 kb on + strand, within BPHYT_RS03140 at 702.270 kb on + strand, within BPHYT_RS03140 at 702.271 kb on - strand, within BPHYT_RS03140 at 702.271 kb on - strand, within BPHYT_RS03140 at 702.271 kb on - strand, within BPHYT_RS03140 at 702.631 kb on + strand, within BPHYT_RS03140 at 702.632 kb on - strand, within BPHYT_RS03140 at 702.828 kb on - strand, within BPHYT_RS03140 at 702.851 kb on + strand at 702.954 kb on + strand at 702.954 kb on + strand at 702.955 kb on - strand at 702.955 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 700,225 + BPHYT_RS03125 0.70 -1.5 700,360 + BPHYT_RS03125 0.79 -0.2 700,360 + BPHYT_RS03125 0.79 -0.1 700,360 + BPHYT_RS03125 0.79 +0.3 700,360 + BPHYT_RS03125 0.79 -0.6 700,433 - BPHYT_RS03125 0.84 -0.7 700,433 - BPHYT_RS03125 0.84 +0.1 700,461 + BPHYT_RS03125 0.86 -1.1 700,735 + -0.3 700,736 - -1.2 700,759 - -0.1 700,759 - -1.8 700,796 + BPHYT_RS03130 0.12 -3.0 701,031 + BPHYT_RS03130 0.73 -0.7 701,115 + +0.1 701,116 - +1.0 701,117 + -0.8 701,117 + +0.4 701,118 - -0.0 701,212 - +0.2 701,212 - +0.2 701,253 + -0.3 701,254 - -4.1 701,297 - BPHYT_RS03135 0.11 +0.1 701,298 + BPHYT_RS03135 0.11 -0.1 701,307 + BPHYT_RS03135 0.12 -1.0 701,307 + BPHYT_RS03135 0.12 -0.4 701,364 - BPHYT_RS03135 0.19 -1.8 701,580 + BPHYT_RS03135 0.45 +0.3 701,581 - BPHYT_RS03135 0.45 -0.6 701,581 - BPHYT_RS03135 0.45 +1.1 701,581 - BPHYT_RS03135 0.45 -0.9 701,581 - BPHYT_RS03135 0.45 -0.7 701,772 + BPHYT_RS03135 0.68 -1.5 701,880 + BPHYT_RS03135 0.81 -0.6 701,880 + BPHYT_RS03135 0.81 -1.0 701,881 - BPHYT_RS03135 0.81 -0.1 701,881 - BPHYT_RS03135 0.81 -0.6 701,982 + -0.4 701,983 - +0.1 702,265 - BPHYT_RS03140 0.26 -0.1 702,270 + BPHYT_RS03140 0.26 -0.4 702,270 + BPHYT_RS03140 0.26 -1.1 702,270 + BPHYT_RS03140 0.26 -0.1 702,271 - BPHYT_RS03140 0.27 -0.7 702,271 - BPHYT_RS03140 0.27 +0.6 702,271 - BPHYT_RS03140 0.27 -0.6 702,631 + BPHYT_RS03140 0.66 -0.6 702,632 - BPHYT_RS03140 0.67 +0.2 702,828 - BPHYT_RS03140 0.88 -0.8 702,851 + -1.4 702,954 + -0.5 702,954 + -0.0 702,955 - +0.5 702,955 - -0.8
Or see this region's nucleotide sequence