Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01040 and BPHYT_RS01045 are separated by 192 nucleotides BPHYT_RS01045 and BPHYT_RS01050 are separated by 446 nucleotides BPHYT_RS01050 and BPHYT_RS01055 are separated by 86 nucleotides
BPHYT_RS01040: BPHYT_RS01040 - Fis family transcriptional regulator, at 238,386 to 238,841
_RS01040
BPHYT_RS01045: BPHYT_RS01045 - hypothetical protein, at 239,034 to 239,327
_RS01045
BPHYT_RS01050: BPHYT_RS01050 - short-chain dehydrogenase, at 239,774 to 240,649
_RS01050
BPHYT_RS01055: BPHYT_RS01055 - LysR family transcriptional regulator, at 240,736 to 241,665
_RS01055
Position (kb) 239
240
241 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 238.844 kb on + strand at 238.845 kb on - strand at 238.845 kb on - strand at 238.845 kb on - strand at 238.849 kb on - strand at 238.849 kb on - strand at 238.895 kb on + strand at 238.899 kb on + strand at 238.900 kb on - strand at 238.904 kb on + strand at 238.905 kb on - strand at 238.905 kb on - strand at 238.912 kb on - strand at 238.980 kb on + strand at 238.980 kb on + strand at 238.995 kb on + strand at 238.996 kb on - strand at 239.212 kb on + strand, within BPHYT_RS01045 at 239.212 kb on + strand, within BPHYT_RS01045 at 239.213 kb on - strand, within BPHYT_RS01045 at 239.213 kb on - strand, within BPHYT_RS01045 at 239.213 kb on - strand, within BPHYT_RS01045 at 239.213 kb on - strand, within BPHYT_RS01045 at 239.296 kb on + strand, within BPHYT_RS01045 at 239.302 kb on + strand at 239.302 kb on + strand at 239.302 kb on + strand at 239.303 kb on - strand at 239.303 kb on - strand at 239.325 kb on + strand at 239.339 kb on + strand at 239.339 kb on + strand at 239.340 kb on - strand at 239.340 kb on - strand at 239.424 kb on - strand at 239.434 kb on + strand at 239.434 kb on + strand at 239.762 kb on + strand at 239.785 kb on + strand at 239.785 kb on + strand at 239.786 kb on - strand at 239.786 kb on - strand at 239.876 kb on - strand, within BPHYT_RS01050 at 239.961 kb on + strand, within BPHYT_RS01050 at 239.962 kb on - strand, within BPHYT_RS01050 at 240.001 kb on - strand, within BPHYT_RS01050 at 240.133 kb on - strand, within BPHYT_RS01050 at 240.447 kb on - strand, within BPHYT_RS01050 at 240.451 kb on + strand, within BPHYT_RS01050 at 240.451 kb on + strand, within BPHYT_RS01050 at 240.452 kb on - strand, within BPHYT_RS01050 at 240.581 kb on + strand at 240.581 kb on + strand at 240.581 kb on + strand at 240.706 kb on - strand at 240.706 kb on - strand at 240.750 kb on - strand at 240.768 kb on + strand at 241.096 kb on + strand, within BPHYT_RS01055 at 241.182 kb on + strand, within BPHYT_RS01055 at 241.183 kb on - strand, within BPHYT_RS01055 at 241.183 kb on - strand, within BPHYT_RS01055 at 241.183 kb on - strand, within BPHYT_RS01055 at 241.244 kb on - strand, within BPHYT_RS01055 at 241.557 kb on + strand, within BPHYT_RS01055
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 238,844 + +1.3 238,845 - -1.2 238,845 - -0.0 238,845 - +0.2 238,849 - +0.2 238,849 - +0.1 238,895 + -0.7 238,899 + +0.4 238,900 - -2.3 238,904 + +0.3 238,905 - -1.8 238,905 - +0.6 238,912 - -0.6 238,980 + +1.1 238,980 + -2.6 238,995 + -1.7 238,996 - +1.6 239,212 + BPHYT_RS01045 0.61 -1.0 239,212 + BPHYT_RS01045 0.61 -0.1 239,213 - BPHYT_RS01045 0.61 -0.0 239,213 - BPHYT_RS01045 0.61 -0.4 239,213 - BPHYT_RS01045 0.61 -3.0 239,213 - BPHYT_RS01045 0.61 -0.8 239,296 + BPHYT_RS01045 0.89 +1.2 239,302 + +0.1 239,302 + -1.3 239,302 + -0.1 239,303 - +0.3 239,303 - -2.3 239,325 + -0.6 239,339 + +0.0 239,339 + -1.1 239,340 - -0.2 239,340 - -1.0 239,424 - +0.4 239,434 + -1.2 239,434 + -0.3 239,762 + -0.2 239,785 + -2.6 239,785 + -1.1 239,786 - -1.3 239,786 - +0.1 239,876 - BPHYT_RS01050 0.12 +0.6 239,961 + BPHYT_RS01050 0.21 +1.5 239,962 - BPHYT_RS01050 0.21 +1.2 240,001 - BPHYT_RS01050 0.26 +0.7 240,133 - BPHYT_RS01050 0.41 +0.3 240,447 - BPHYT_RS01050 0.77 +0.4 240,451 + BPHYT_RS01050 0.77 -0.3 240,451 + BPHYT_RS01050 0.77 -0.1 240,452 - BPHYT_RS01050 0.77 -1.4 240,581 + -0.5 240,581 + -0.0 240,581 + -0.3 240,706 - -0.5 240,706 - +1.1 240,750 - -0.6 240,768 + +0.2 241,096 + BPHYT_RS01055 0.39 -2.1 241,182 + BPHYT_RS01055 0.48 -0.3 241,183 - BPHYT_RS01055 0.48 +0.7 241,183 - BPHYT_RS01055 0.48 -0.4 241,183 - BPHYT_RS01055 0.48 -0.2 241,244 - BPHYT_RS01055 0.55 -0.5 241,557 + BPHYT_RS01055 0.88 -0.3
Or see this region's nucleotide sequence