Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00240 and BPHYT_RS00245 overlap by 4 nucleotides BPHYT_RS00245 and BPHYT_RS00250 are separated by 170 nucleotides BPHYT_RS00250 and BPHYT_RS00255 are separated by 68 nucleotides
BPHYT_RS00240: BPHYT_RS00240 - amino acid ABC substrate-binding protein, at 62,769 to 63,911
_RS00240
BPHYT_RS00245: BPHYT_RS00245 - response regulator receiver, at 63,908 to 64,510
_RS00245
BPHYT_RS00250: BPHYT_RS00250 - hydratase, at 64,681 to 65,532
_RS00250
BPHYT_RS00255: BPHYT_RS00255 - amino acid ABC transporter, at 65,601 to 66,764
_RS00255
Position (kb) 64
65
66 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 63.764 kb on + strand, within BPHYT_RS00240 at 63.764 kb on + strand, within BPHYT_RS00240 at 63.855 kb on + strand at 63.944 kb on - strand at 63.957 kb on + strand at 63.957 kb on + strand at 63.957 kb on + strand at 64.192 kb on + strand, within BPHYT_RS00245 at 64.584 kb on - strand at 64.587 kb on - strand at 64.757 kb on + strand at 64.758 kb on - strand at 64.942 kb on + strand, within BPHYT_RS00250 at 64.985 kb on - strand, within BPHYT_RS00250 at 65.017 kb on + strand, within BPHYT_RS00250 at 65.099 kb on - strand, within BPHYT_RS00250 at 65.170 kb on + strand, within BPHYT_RS00250 at 65.170 kb on + strand, within BPHYT_RS00250 at 65.170 kb on + strand, within BPHYT_RS00250 at 65.171 kb on - strand, within BPHYT_RS00250 at 65.196 kb on - strand, within BPHYT_RS00250 at 65.217 kb on - strand, within BPHYT_RS00250 at 65.393 kb on + strand, within BPHYT_RS00250 at 65.394 kb on - strand, within BPHYT_RS00250 at 65.497 kb on - strand at 65.500 kb on + strand at 65.501 kb on - strand at 65.597 kb on + strand at 65.597 kb on + strand at 65.597 kb on + strand at 65.598 kb on - strand at 65.598 kb on - strand at 65.612 kb on - strand at 65.862 kb on + strand, within BPHYT_RS00255 at 65.863 kb on - strand, within BPHYT_RS00255 at 65.969 kb on + strand, within BPHYT_RS00255 at 65.970 kb on - strand, within BPHYT_RS00255 at 66.009 kb on + strand, within BPHYT_RS00255 at 66.009 kb on + strand, within BPHYT_RS00255 at 66.009 kb on + strand, within BPHYT_RS00255 at 66.010 kb on - strand, within BPHYT_RS00255 at 66.010 kb on - strand, within BPHYT_RS00255 at 66.010 kb on - strand at 66.010 kb on - strand, within BPHYT_RS00255 at 66.010 kb on - strand, within BPHYT_RS00255 at 66.015 kb on + strand, within BPHYT_RS00255 at 66.015 kb on + strand, within BPHYT_RS00255 at 66.015 kb on + strand at 66.015 kb on + strand, within BPHYT_RS00255 at 66.016 kb on - strand, within BPHYT_RS00255 at 66.016 kb on - strand, within BPHYT_RS00255 at 66.016 kb on - strand, within BPHYT_RS00255 at 66.016 kb on - strand, within BPHYT_RS00255 at 66.017 kb on + strand, within BPHYT_RS00255 at 66.017 kb on + strand, within BPHYT_RS00255 at 66.018 kb on - strand, within BPHYT_RS00255 at 66.064 kb on - strand, within BPHYT_RS00255 at 66.064 kb on - strand, within BPHYT_RS00255 at 66.153 kb on + strand, within BPHYT_RS00255 at 66.153 kb on + strand, within BPHYT_RS00255 at 66.193 kb on - strand, within BPHYT_RS00255 at 66.403 kb on - strand, within BPHYT_RS00255 at 66.420 kb on + strand, within BPHYT_RS00255 at 66.420 kb on + strand, within BPHYT_RS00255 at 66.421 kb on - strand, within BPHYT_RS00255 at 66.522 kb on + strand, within BPHYT_RS00255 at 66.522 kb on + strand, within BPHYT_RS00255
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 63,764 + BPHYT_RS00240 0.87 -0.5 63,764 + BPHYT_RS00240 0.87 -3.3 63,855 + +1.0 63,944 - +0.3 63,957 + +0.4 63,957 + +3.3 63,957 + -1.8 64,192 + BPHYT_RS00245 0.47 -1.0 64,584 - -0.4 64,587 - -0.7 64,757 + +0.4 64,758 - +1.3 64,942 + BPHYT_RS00250 0.31 +0.5 64,985 - BPHYT_RS00250 0.36 -0.8 65,017 + BPHYT_RS00250 0.39 -1.0 65,099 - BPHYT_RS00250 0.49 +0.5 65,170 + BPHYT_RS00250 0.57 +1.1 65,170 + BPHYT_RS00250 0.57 -1.2 65,170 + BPHYT_RS00250 0.57 +2.7 65,171 - BPHYT_RS00250 0.58 -0.6 65,196 - BPHYT_RS00250 0.60 -1.3 65,217 - BPHYT_RS00250 0.63 -0.1 65,393 + BPHYT_RS00250 0.84 -0.8 65,394 - BPHYT_RS00250 0.84 -2.2 65,497 - -1.2 65,500 + -1.5 65,501 - +0.0 65,597 + +0.5 65,597 + +0.0 65,597 + -0.6 65,598 - -1.4 65,598 - +0.3 65,612 - -0.3 65,862 + BPHYT_RS00255 0.22 -2.5 65,863 - BPHYT_RS00255 0.23 -0.9 65,969 + BPHYT_RS00255 0.32 -0.4 65,970 - BPHYT_RS00255 0.32 +0.8 66,009 + BPHYT_RS00255 0.35 +2.1 66,009 + BPHYT_RS00255 0.35 -0.4 66,009 + BPHYT_RS00255 0.35 -1.1 66,010 - BPHYT_RS00255 0.35 -0.0 66,010 - BPHYT_RS00255 0.35 +0.0 66,010 - +1.1 66,010 - BPHYT_RS00255 0.35 -2.5 66,010 - BPHYT_RS00255 0.35 -0.2 66,015 + BPHYT_RS00255 0.36 -0.4 66,015 + BPHYT_RS00255 0.36 -1.7 66,015 + -0.1 66,015 + BPHYT_RS00255 0.36 +0.3 66,016 - BPHYT_RS00255 0.36 +0.3 66,016 - BPHYT_RS00255 0.36 -0.9 66,016 - BPHYT_RS00255 0.36 +2.7 66,016 - BPHYT_RS00255 0.36 +0.4 66,017 + BPHYT_RS00255 0.36 +0.8 66,017 + BPHYT_RS00255 0.36 +0.2 66,018 - BPHYT_RS00255 0.36 -1.2 66,064 - BPHYT_RS00255 0.40 +0.1 66,064 - BPHYT_RS00255 0.40 +0.3 66,153 + BPHYT_RS00255 0.47 +0.8 66,153 + BPHYT_RS00255 0.47 +0.4 66,193 - BPHYT_RS00255 0.51 -1.7 66,403 - BPHYT_RS00255 0.69 -0.5 66,420 + BPHYT_RS00255 0.70 -0.6 66,420 + BPHYT_RS00255 0.70 +0.3 66,421 - BPHYT_RS00255 0.70 -2.4 66,522 + BPHYT_RS00255 0.79 -0.3 66,522 + BPHYT_RS00255 0.79 +1.3
Or see this region's nucleotide sequence