Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36460 and BPHYT_RS36465 are separated by 58 nucleotides BPHYT_RS36465 and BPHYT_RS36470 are separated by 252 nucleotides BPHYT_RS36470 and BPHYT_RS36475 are separated by 431 nucleotides
BPHYT_RS36460: BPHYT_RS36460 - hypothetical protein, at 102,791 to 103,294
_RS36460
BPHYT_RS36465: BPHYT_RS36465 - DNA-binding protein, at 103,353 to 103,673
_RS36465
BPHYT_RS36470: BPHYT_RS36470 - hypothetical protein, at 103,926 to 104,846
_RS36470
BPHYT_RS36475: BPHYT_RS36475 - hypothetical protein, at 105,278 to 105,775
_RS36475
Position (kb) 103
104
105 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 103.020 kb on - strand, within BPHYT_RS36460 at 103.023 kb on - strand, within BPHYT_RS36460 at 103.261 kb on - strand at 103.261 kb on - strand at 103.293 kb on - strand at 103.322 kb on - strand at 103.482 kb on + strand, within BPHYT_RS36465 at 103.749 kb on + strand at 103.749 kb on + strand at 103.750 kb on - strand at 103.939 kb on + strand at 103.939 kb on - strand at 103.939 kb on - strand at 103.989 kb on + strand at 104.014 kb on - strand at 104.081 kb on + strand, within BPHYT_RS36470 at 104.082 kb on - strand, within BPHYT_RS36470 at 104.986 kb on + strand at 105.113 kb on - strand at 105.210 kb on + strand at 105.213 kb on + strand at 105.279 kb on - strand at 105.368 kb on - strand, within BPHYT_RS36475 at 105.462 kb on - strand, within BPHYT_RS36475 at 105.617 kb on + strand, within BPHYT_RS36475 at 105.618 kb on - strand, within BPHYT_RS36475
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 103,020 - BPHYT_RS36460 0.45 +1.2 103,023 - BPHYT_RS36460 0.46 -4.3 103,261 - +1.3 103,261 - +1.9 103,293 - -2.3 103,322 - +1.2 103,482 + BPHYT_RS36465 0.40 +0.9 103,749 + -2.1 103,749 + +0.3 103,750 - -1.5 103,939 + +0.9 103,939 - +0.6 103,939 - -1.1 103,989 + -2.5 104,014 - +0.3 104,081 + BPHYT_RS36470 0.17 -2.3 104,082 - BPHYT_RS36470 0.17 -2.5 104,986 + -0.7 105,113 - -0.9 105,210 + -0.4 105,213 + -1.3 105,279 - -1.6 105,368 - BPHYT_RS36475 0.18 +0.3 105,462 - BPHYT_RS36475 0.37 -0.1 105,617 + BPHYT_RS36475 0.68 +1.5 105,618 - BPHYT_RS36475 0.68 +0.3
Or see this region's nucleotide sequence