Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33805

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS33800 and BPHYT_RS33805 are separated by 181 nucleotidesBPHYT_RS33805 and BPHYT_RS33810 overlap by 19 nucleotidesBPHYT_RS33810 and BPHYT_RS33815 are separated by 59 nucleotides BPHYT_RS33800: BPHYT_RS33800 - glycosyl hydrolase, at 3,174,620 to 3,177,475 _RS33800 BPHYT_RS33805: BPHYT_RS33805 - hypothetical protein, at 3,177,657 to 3,177,866 _RS33805 BPHYT_RS33810: BPHYT_RS33810 - membrane protein, at 3,177,848 to 3,178,570 _RS33810 BPHYT_RS33815: BPHYT_RS33815 - hypothetical protein, at 3,178,630 to 3,179,973 _RS33815 Position (kb) 3177 3178Strain fitness (log2 ratio) -3 -2 -1 0 1at 3177.153 kb on - strand, within BPHYT_RS33800at 3177.407 kb on + strandat 3177.510 kb on + strandat 3177.788 kb on - strand, within BPHYT_RS33805at 3178.114 kb on - strand, within BPHYT_RS33810at 3178.114 kb on - strand, within BPHYT_RS33810at 3178.356 kb on + strand, within BPHYT_RS33810at 3178.599 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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3,177,153 - BPHYT_RS33800 0.89 +0.2
3,177,407 + +0.1
3,177,510 + +0.4
3,177,788 - BPHYT_RS33805 0.62 -3.1
3,178,114 - BPHYT_RS33810 0.37 -1.9
3,178,114 - BPHYT_RS33810 0.37 -0.1
3,178,356 + BPHYT_RS33810 0.70 +0.1
3,178,599 - -0.4

Or see this region's nucleotide sequence