Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32855 and BPHYT_RS32860 are separated by 0 nucleotides BPHYT_RS32860 and BPHYT_RS32865 are separated by 307 nucleotides BPHYT_RS32865 and BPHYT_RS32870 are separated by 207 nucleotides BPHYT_RS32870 and BPHYT_RS32875 are separated by 206 nucleotides
BPHYT_RS32855: BPHYT_RS32855 - TetR family transcriptional regulator, at 2,951,980 to 2,952,474
_RS32855
BPHYT_RS32860: BPHYT_RS32860 - hypothetical protein, at 2,952,475 to 2,952,702
_RS32860
BPHYT_RS32865: BPHYT_RS32865 - choloylglycine hydrolase, at 2,953,010 to 2,954,089
_RS32865
BPHYT_RS32870: BPHYT_RS32870 - hypothetical protein, at 2,954,297 to 2,954,743
_RS32870
BPHYT_RS32875: BPHYT_RS32875 - GCN5 family acetyltransferase, at 2,954,950 to 2,955,762
_RS32875
Position (kb)
2953
2954
2955 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2952.635 kb on - strand, within BPHYT_RS32860 at 2952.889 kb on - strand at 2952.999 kb on - strand at 2953.104 kb on + strand at 2953.107 kb on - strand at 2953.731 kb on - strand, within BPHYT_RS32865 at 2953.734 kb on + strand, within BPHYT_RS32865 at 2953.823 kb on - strand, within BPHYT_RS32865 at 2954.006 kb on - strand at 2954.414 kb on + strand, within BPHYT_RS32870 at 2954.524 kb on - strand, within BPHYT_RS32870 at 2954.660 kb on + strand, within BPHYT_RS32870 at 2954.773 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,952,635 - BPHYT_RS32860 0.70 -3.1 2,952,889 - -1.5 2,952,999 - -0.8 2,953,104 + -0.4 2,953,107 - +0.5 2,953,731 - BPHYT_RS32865 0.67 +0.1 2,953,734 + BPHYT_RS32865 0.67 -0.2 2,953,823 - BPHYT_RS32865 0.75 -0.2 2,954,006 - +0.6 2,954,414 + BPHYT_RS32870 0.26 -0.7 2,954,524 - BPHYT_RS32870 0.51 +1.2 2,954,660 + BPHYT_RS32870 0.81 +0.1 2,954,773 + -0.1
Or see this region's nucleotide sequence