Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30765 and BPHYT_RS30770 are separated by 100 nucleotides BPHYT_RS30770 and BPHYT_RS30775 are separated by 105 nucleotides BPHYT_RS30775 and BPHYT_RS30780 are separated by 60 nucleotides
BPHYT_RS30765: BPHYT_RS30765 - hypothetical protein, at 2,457,794 to 2,458,531
_RS30765
BPHYT_RS30770: BPHYT_RS30770 - hypothetical protein, at 2,458,632 to 2,459,177
_RS30770
BPHYT_RS30775: BPHYT_RS30775 - gluconate 2-dehydrogenase, at 2,459,283 to 2,460,038
_RS30775
BPHYT_RS30780: BPHYT_RS30780 - GMC family oxidoreductase, at 2,460,099 to 2,461,883
_RS30780
Position (kb)
2459
2460
2461 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2458.631 kb on + strand at 2458.998 kb on + strand, within BPHYT_RS30770 at 2458.999 kb on - strand, within BPHYT_RS30770 at 2458.999 kb on - strand, within BPHYT_RS30770 at 2459.025 kb on + strand, within BPHYT_RS30770 at 2459.701 kb on - strand, within BPHYT_RS30775 at 2459.701 kb on - strand, within BPHYT_RS30775 at 2459.968 kb on - strand at 2460.800 kb on - strand, within BPHYT_RS30780 at 2460.843 kb on + strand, within BPHYT_RS30780 at 2460.984 kb on - strand, within BPHYT_RS30780
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,458,631 + -1.4 2,458,998 + BPHYT_RS30770 0.67 -0.4 2,458,999 - BPHYT_RS30770 0.67 +0.1 2,458,999 - BPHYT_RS30770 0.67 +0.5 2,459,025 + BPHYT_RS30770 0.72 +0.1 2,459,701 - BPHYT_RS30775 0.55 -2.7 2,459,701 - BPHYT_RS30775 0.55 -2.8 2,459,968 - +1.5 2,460,800 - BPHYT_RS30780 0.39 +1.6 2,460,843 + BPHYT_RS30780 0.42 +1.9 2,460,984 - BPHYT_RS30780 0.50 -0.3
Or see this region's nucleotide sequence