Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29795 and BPHYT_RS29800 are separated by 229 nucleotides BPHYT_RS29800 and BPHYT_RS29805 are separated by 113 nucleotides BPHYT_RS29805 and BPHYT_RS29810 are separated by 13 nucleotides BPHYT_RS29810 and BPHYT_RS29815 are separated by 43 nucleotides
BPHYT_RS29795: BPHYT_RS29795 - elongation factor, at 2,238,011 to 2,238,595
_RS29795
BPHYT_RS29800: BPHYT_RS29800 - hypothetical protein, at 2,238,825 to 2,239,364
_RS29800
BPHYT_RS29805: BPHYT_RS29805 - lysine transporter LysE, at 2,239,478 to 2,240,098
_RS29805
BPHYT_RS29810: BPHYT_RS29810 - hexapeptide transferase, at 2,240,112 to 2,240,531
_RS29810
BPHYT_RS29815: BPHYT_RS29815 - UDP-N-acetyl-D-mannosamine transferase, at 2,240,575 to 2,241,309
_RS29815
Position (kb)
2239
2240
2241 Strain fitness (log2 ratio)
-2
-1
0
1 at 2238.642 kb on + strand at 2238.671 kb on - strand at 2238.705 kb on + strand at 2238.706 kb on - strand at 2238.708 kb on + strand at 2238.708 kb on + strand at 2238.712 kb on + strand at 2238.712 kb on + strand at 2238.713 kb on - strand at 2238.899 kb on + strand, within BPHYT_RS29800 at 2239.301 kb on + strand, within BPHYT_RS29800 at 2239.484 kb on - strand at 2239.970 kb on + strand, within BPHYT_RS29805 at 2239.971 kb on - strand, within BPHYT_RS29805 at 2239.989 kb on - strand, within BPHYT_RS29805 at 2240.314 kb on - strand, within BPHYT_RS29810 at 2240.546 kb on + strand at 2240.547 kb on - strand at 2240.996 kb on + strand, within BPHYT_RS29815
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,238,642 + -1.8 2,238,671 - -0.5 2,238,705 + -1.2 2,238,706 - +0.8 2,238,708 + -0.5 2,238,708 + +0.4 2,238,712 + -2.1 2,238,712 + -1.0 2,238,713 - +0.9 2,238,899 + BPHYT_RS29800 0.14 -0.7 2,239,301 + BPHYT_RS29800 0.88 -0.7 2,239,484 - -0.3 2,239,970 + BPHYT_RS29805 0.79 -0.6 2,239,971 - BPHYT_RS29805 0.79 -1.0 2,239,989 - BPHYT_RS29805 0.82 -0.7 2,240,314 - BPHYT_RS29810 0.48 +0.7 2,240,546 + +1.2 2,240,547 - -1.8 2,240,996 + BPHYT_RS29815 0.57 +0.2
Or see this region's nucleotide sequence