Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29485

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29475 and BPHYT_RS29480 overlap by 4 nucleotidesBPHYT_RS29480 and BPHYT_RS29485 are separated by 8 nucleotidesBPHYT_RS29485 and BPHYT_RS29490 are separated by 119 nucleotides BPHYT_RS29475: BPHYT_RS29475 - carbon-phosphorus lyase complex subunit PhnJ, at 2,165,335 to 2,166,267 _RS29475 BPHYT_RS29480: BPHYT_RS29480 - phosphonate C-P lyase, at 2,166,264 to 2,167,034 _RS29480 BPHYT_RS29485: BPHYT_RS29485 - phosphonate ABC transporter ATP-binding protein, at 2,167,043 to 2,167,807 _RS29485 BPHYT_RS29490: BPHYT_RS29490 - phosphonate metabolism protein PhnM, at 2,167,927 to 2,169,060 _RS29490 Position (kb) 2167 2168Strain fitness (log2 ratio) -2 -1 0 1at 2167.273 kb on - strand, within BPHYT_RS29485at 2167.597 kb on + strand, within BPHYT_RS29485at 2167.598 kb on - strand, within BPHYT_RS29485at 2167.817 kb on - strandat 2167.817 kb on - strandat 2167.968 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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2,167,273 - BPHYT_RS29485 0.30 -1.0
2,167,597 + BPHYT_RS29485 0.72 +0.1
2,167,598 - BPHYT_RS29485 0.73 -1.4
2,167,817 - -0.4
2,167,817 - -2.2
2,167,968 - -2.3

Or see this region's nucleotide sequence