Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29095 and BPHYT_RS29100 are separated by 191 nucleotides BPHYT_RS29100 and BPHYT_RS29105 are separated by 15 nucleotides BPHYT_RS29105 and BPHYT_RS29110 are separated by 245 nucleotides
BPHYT_RS29095: BPHYT_RS29095 - coenzyme F420-dependent NADP oxidoreductase, at 2,081,689 to 2,082,288
_RS29095
BPHYT_RS29100: BPHYT_RS29100 - ketosteroid isomerase, at 2,082,480 to 2,082,896
_RS29100
BPHYT_RS29105: BPHYT_RS29105 - class V aminotransferase, at 2,082,912 to 2,084,039
_RS29105
BPHYT_RS29110: BPHYT_RS29110 - DNA-directed DNA polymerase, at 2,084,285 to 2,085,430
_RS29110
Position (kb)
2082
2083
2084
2085 Strain fitness (log2 ratio)
-2
-1
0
1 at 2082.096 kb on - strand, within BPHYT_RS29095 at 2082.837 kb on + strand, within BPHYT_RS29100 at 2082.913 kb on + strand at 2083.234 kb on + strand, within BPHYT_RS29105 at 2083.691 kb on - strand, within BPHYT_RS29105 at 2083.901 kb on - strand, within BPHYT_RS29105 at 2083.958 kb on + strand at 2083.959 kb on - strand at 2084.064 kb on - strand at 2084.064 kb on - strand at 2084.065 kb on + strand at 2084.211 kb on + strand at 2084.230 kb on - strand at 2084.305 kb on - strand at 2084.377 kb on + strand at 2084.779 kb on + strand, within BPHYT_RS29110 at 2084.779 kb on + strand, within BPHYT_RS29110
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 2,082,096 - BPHYT_RS29095 0.68 +0.9 2,082,837 + BPHYT_RS29100 0.86 -0.4 2,082,913 + +0.5 2,083,234 + BPHYT_RS29105 0.29 -2.0 2,083,691 - BPHYT_RS29105 0.69 -0.4 2,083,901 - BPHYT_RS29105 0.88 -2.2 2,083,958 + -1.4 2,083,959 - +0.2 2,084,064 - -0.0 2,084,064 - -0.2 2,084,065 + -0.0 2,084,211 + -1.6 2,084,230 - -0.8 2,084,305 - +0.0 2,084,377 + +0.6 2,084,779 + BPHYT_RS29110 0.43 -0.1 2,084,779 + BPHYT_RS29110 0.43 -1.9
Or see this region's nucleotide sequence