Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25545

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25540 and BPHYT_RS25545 are separated by 347 nucleotidesBPHYT_RS25545 and BPHYT_RS25550 are separated by 188 nucleotides BPHYT_RS25540: BPHYT_RS25540 - phosphohydrolase, at 1,302,614 to 1,303,438 _RS25540 BPHYT_RS25545: BPHYT_RS25545 - hypothetical protein, at 1,303,786 to 1,305,726 _RS25545 BPHYT_RS25550: BPHYT_RS25550 - hypothetical protein, at 1,305,915 to 1,306,607 _RS25550 Position (kb) 1303 1304 1305 1306Strain fitness (log2 ratio) -2 -1 0 1at 1302.913 kb on - strand, within BPHYT_RS25540at 1302.998 kb on - strand, within BPHYT_RS25540at 1303.374 kb on + strandat 1303.418 kb on - strandat 1303.418 kb on - strandat 1303.859 kb on - strandat 1303.954 kb on - strandat 1305.468 kb on + strand, within BPHYT_RS25545at 1305.468 kb on + strand, within BPHYT_RS25545at 1305.501 kb on - strand, within BPHYT_RS25545

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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1,302,913 - BPHYT_RS25540 0.36 +0.1
1,302,998 - BPHYT_RS25540 0.47 -2.6
1,303,374 + +1.6
1,303,418 - -1.5
1,303,418 - -0.7
1,303,859 - -0.9
1,303,954 - -1.9
1,305,468 + BPHYT_RS25545 0.87 -1.2
1,305,468 + BPHYT_RS25545 0.87 -1.4
1,305,501 - BPHYT_RS25545 0.88 -1.7

Or see this region's nucleotide sequence