Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25330 and BPHYT_RS25335 are separated by 284 nucleotides BPHYT_RS25335 and BPHYT_RS25340 are separated by 124 nucleotides BPHYT_RS25340 and BPHYT_RS25345 are separated by 56 nucleotides
BPHYT_RS25330: BPHYT_RS25330 - hypothetical protein, at 1,257,215 to 1,258,597
_RS25330
BPHYT_RS25335: BPHYT_RS25335 - hypothetical protein, at 1,258,882 to 1,259,196
_RS25335
BPHYT_RS25340: BPHYT_RS25340 - sulfatase, at 1,259,321 to 1,260,448
_RS25340
BPHYT_RS25345: BPHYT_RS25345 - arylsulfatase, at 1,260,505 to 1,262,157
_RS25345
Position (kb) 1259
1260
1261 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1258.642 kb on + strand at 1258.710 kb on - strand at 1258.715 kb on - strand at 1258.810 kb on - strand at 1258.888 kb on + strand at 1258.897 kb on - strand at 1258.934 kb on - strand, within BPHYT_RS25335 at 1259.428 kb on - strand at 1259.592 kb on + strand, within BPHYT_RS25340 at 1259.634 kb on + strand, within BPHYT_RS25340 at 1259.896 kb on - strand, within BPHYT_RS25340 at 1260.536 kb on + strand at 1260.791 kb on + strand, within BPHYT_RS25345 at 1260.864 kb on - strand, within BPHYT_RS25345 at 1261.226 kb on + strand, within BPHYT_RS25345 at 1261.227 kb on - strand, within BPHYT_RS25345 at 1261.272 kb on - strand, within BPHYT_RS25345 at 1261.415 kb on - strand, within BPHYT_RS25345
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 1,258,642 + -0.1 1,258,710 - -0.0 1,258,715 - -0.2 1,258,810 - +1.0 1,258,888 + +0.3 1,258,897 - +0.5 1,258,934 - BPHYT_RS25335 0.17 +0.2 1,259,428 - -1.7 1,259,592 + BPHYT_RS25340 0.24 -3.9 1,259,634 + BPHYT_RS25340 0.28 -0.9 1,259,896 - BPHYT_RS25340 0.51 -0.7 1,260,536 + -1.8 1,260,791 + BPHYT_RS25345 0.17 -1.0 1,260,864 - BPHYT_RS25345 0.22 -0.1 1,261,226 + BPHYT_RS25345 0.44 -1.1 1,261,227 - BPHYT_RS25345 0.44 +0.5 1,261,272 - BPHYT_RS25345 0.46 -1.1 1,261,415 - BPHYT_RS25345 0.55 -0.8
Or see this region's nucleotide sequence