Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS25125

Experiment: P. phyto PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS25115 and BPHYT_RS25120 overlap by 11 nucleotidesBPHYT_RS25120 and BPHYT_RS25125 are separated by 57 nucleotidesBPHYT_RS25125 and BPHYT_RS25130 are separated by 142 nucleotides BPHYT_RS25115: BPHYT_RS25115 - sarcosine oxidase subunit alpha, at 1,206,681 to 1,209,683 _RS25115 BPHYT_RS25120: BPHYT_RS25120 - sarcosine oxidase subunit gamma, at 1,209,673 to 1,210,329 _RS25120 BPHYT_RS25125: BPHYT_RS25125 - hypothetical protein, at 1,210,387 to 1,210,650 _RS25125 BPHYT_RS25130: BPHYT_RS25130 - MFS transporter, at 1,210,793 to 1,212,211 _RS25130 Position (kb) 1210 1211Strain fitness (log2 ratio) -3 -2 -1 0 1at 1209.392 kb on + strandat 1210.282 kb on + strandat 1210.711 kb on + strandat 1210.712 kb on - strandat 1211.284 kb on + strand, within BPHYT_RS25130at 1211.364 kb on + strand, within BPHYT_RS25130

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Per-strain Table

Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1
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1,209,392 + -2.8
1,210,282 + -0.5
1,210,711 + -2.1
1,210,712 - -3.6
1,211,284 + BPHYT_RS25130 0.35 -0.3
1,211,364 + BPHYT_RS25130 0.40 +0.1

Or see this region's nucleotide sequence