Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24730 and BPHYT_RS24735 are separated by 93 nucleotides BPHYT_RS24735 and BPHYT_RS24740 overlap by 4 nucleotides BPHYT_RS24740 and BPHYT_RS24745 are separated by 148 nucleotides
BPHYT_RS24730: BPHYT_RS24730 - AraC family transcriptional regulator, at 1,114,729 to 1,115,685
_RS24730
BPHYT_RS24735: BPHYT_RS24735 - transcriptional regulator, at 1,115,779 to 1,116,606
_RS24735
BPHYT_RS24740: BPHYT_RS24740 - RNA polymerase subunit sigma-24, at 1,116,603 to 1,117,121
_RS24740
BPHYT_RS24745: BPHYT_RS24745 - transcriptional regulator, at 1,117,270 to 1,117,992
_RS24745
Position (kb) 1116
1117
1118 Strain fitness (log2 ratio) -2
-1
0
1 at 1115.634 kb on + strand at 1116.734 kb on - strand, within BPHYT_RS24740 at 1116.734 kb on - strand, within BPHYT_RS24740 at 1116.802 kb on + strand, within BPHYT_RS24740 at 1117.256 kb on + strand at 1117.257 kb on - strand at 1117.257 kb on - strand at 1117.470 kb on - strand, within BPHYT_RS24745 at 1117.848 kb on - strand, within BPHYT_RS24745 at 1118.051 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 1,115,634 + -1.6 1,116,734 - BPHYT_RS24740 0.25 +0.3 1,116,734 - BPHYT_RS24740 0.25 +0.9 1,116,802 + BPHYT_RS24740 0.38 +0.7 1,117,256 + +1.2 1,117,257 - -1.4 1,117,257 - -2.2 1,117,470 - BPHYT_RS24745 0.28 -0.5 1,117,848 - BPHYT_RS24745 0.80 -2.4 1,118,051 + -1.8
Or see this region's nucleotide sequence