Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24500 and BPHYT_RS24505 overlap by 4 nucleotides BPHYT_RS24505 and BPHYT_RS24510 are separated by 54 nucleotides BPHYT_RS24510 and BPHYT_RS24515 are separated by 267 nucleotides
BPHYT_RS24500: BPHYT_RS24500 - histidine kinase, at 1,064,744 to 1,066,144
_RS24500
BPHYT_RS24505: BPHYT_RS24505 - glyoxalase, at 1,066,141 to 1,066,566
_RS24505
BPHYT_RS24510: BPHYT_RS24510 - alkylphosphonate utilization protein, at 1,066,621 to 1,066,962
_RS24510
BPHYT_RS24515: BPHYT_RS24515 - antibiotic biosynthesis monooxygenase, at 1,067,230 to 1,067,532
_RS24515
Position (kb)
1066
1067 Strain fitness (log2 ratio)
-2
-1
0
1 at 1065.473 kb on + strand, within BPHYT_RS24500 at 1065.587 kb on + strand, within BPHYT_RS24500 at 1066.142 kb on + strand at 1066.230 kb on - strand, within BPHYT_RS24505 at 1066.242 kb on - strand, within BPHYT_RS24505 at 1066.512 kb on - strand, within BPHYT_RS24505 at 1066.586 kb on + strand at 1066.898 kb on + strand, within BPHYT_RS24510 at 1066.898 kb on + strand, within BPHYT_RS24510 at 1066.899 kb on - strand, within BPHYT_RS24510 at 1067.116 kb on + strand at 1067.134 kb on + strand at 1067.135 kb on - strand at 1067.135 kb on - strand at 1067.144 kb on + strand at 1067.249 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 1,065,473 + BPHYT_RS24500 0.52 -0.4 1,065,587 + BPHYT_RS24500 0.60 +1.1 1,066,142 + -0.0 1,066,230 - BPHYT_RS24505 0.21 -1.8 1,066,242 - BPHYT_RS24505 0.24 -1.5 1,066,512 - BPHYT_RS24505 0.87 -1.9 1,066,586 + -0.8 1,066,898 + BPHYT_RS24510 0.81 +0.6 1,066,898 + BPHYT_RS24510 0.81 -1.3 1,066,899 - BPHYT_RS24510 0.81 -0.7 1,067,116 + -1.0 1,067,134 + -0.1 1,067,135 - +1.2 1,067,135 - +0.0 1,067,144 + -0.8 1,067,249 - -0.6
Or see this region's nucleotide sequence