Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23635 and BPHYT_RS23640 are separated by 86 nucleotides BPHYT_RS23640 and BPHYT_RS23645 are separated by 113 nucleotides BPHYT_RS23645 and BPHYT_RS23650 are separated by 160 nucleotides
BPHYT_RS23635: BPHYT_RS23635 - phosphoesterase, at 872,681 to 874,687
_RS23635
BPHYT_RS23640: BPHYT_RS23640 - hypothetical protein, at 874,774 to 875,472
_RS23640
BPHYT_RS23645: BPHYT_RS23645 - phosphohydrolase, at 875,586 to 876,230
_RS23645
BPHYT_RS23650: BPHYT_RS23650 - hypothetical protein, at 876,391 to 876,729
_RS23650
Position (kb) 874
875
876 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 873.853 kb on - strand, within BPHYT_RS23635 at 874.437 kb on + strand, within BPHYT_RS23635 at 874.438 kb on - strand, within BPHYT_RS23635 at 874.438 kb on - strand, within BPHYT_RS23635 at 874.665 kb on - strand at 874.665 kb on - strand at 874.674 kb on + strand at 874.726 kb on - strand at 874.860 kb on - strand, within BPHYT_RS23640 at 874.892 kb on + strand, within BPHYT_RS23640 at 874.893 kb on - strand, within BPHYT_RS23640 at 875.073 kb on + strand, within BPHYT_RS23640 at 875.074 kb on - strand, within BPHYT_RS23640 at 875.138 kb on + strand, within BPHYT_RS23640 at 875.501 kb on + strand at 875.560 kb on - strand at 875.770 kb on + strand, within BPHYT_RS23645 at 875.770 kb on + strand, within BPHYT_RS23645 at 875.771 kb on - strand, within BPHYT_RS23645 at 876.037 kb on - strand, within BPHYT_RS23645 at 876.170 kb on - strand at 876.260 kb on + strand at 876.324 kb on + strand at 876.325 kb on - strand at 876.327 kb on + strand at 876.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 873,853 - BPHYT_RS23635 0.58 -0.4 874,437 + BPHYT_RS23635 0.87 +1.0 874,438 - BPHYT_RS23635 0.88 -2.0 874,438 - BPHYT_RS23635 0.88 -0.4 874,665 - -1.1 874,665 - +0.2 874,674 + +0.1 874,726 - -0.1 874,860 - BPHYT_RS23640 0.12 -0.1 874,892 + BPHYT_RS23640 0.17 +0.0 874,893 - BPHYT_RS23640 0.17 -0.6 875,073 + BPHYT_RS23640 0.43 -2.7 875,074 - BPHYT_RS23640 0.43 +0.6 875,138 + BPHYT_RS23640 0.52 -0.4 875,501 + -2.4 875,560 - +0.1 875,770 + BPHYT_RS23645 0.29 -0.7 875,770 + BPHYT_RS23645 0.29 -2.3 875,771 - BPHYT_RS23645 0.29 -1.3 876,037 - BPHYT_RS23645 0.70 -0.6 876,170 - +1.3 876,260 + -3.0 876,324 + -1.0 876,325 - +0.4 876,327 + +0.7 876,412 - +0.1
Or see this region's nucleotide sequence