Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23045 and BPHYT_RS23050 are separated by 131 nucleotides BPHYT_RS23050 and BPHYT_RS23055 are separated by 272 nucleotides BPHYT_RS23055 and BPHYT_RS23060 are separated by 201 nucleotides
BPHYT_RS23045: BPHYT_RS23045 - AsnC family transcriptional regulator, at 732,189 to 732,854
_RS23045
BPHYT_RS23050: BPHYT_RS23050 - hypothetical protein, at 732,986 to 733,306
_RS23050
BPHYT_RS23055: BPHYT_RS23055 - LysR family transcriptional regulator, at 733,579 to 734,481
_RS23055
BPHYT_RS23060: BPHYT_RS23060 - 3-isopropylmalate dehydratase large subunit, at 734,683 to 736,650
_RS23060
Position (kb) 733
734
735 Strain fitness (log2 ratio) -2
-1
0
1
2 at 733.046 kb on + strand, within BPHYT_RS23050 at 733.433 kb on + strand at 733.434 kb on - strand at 733.549 kb on + strand at 734.536 kb on + strand at 734.660 kb on + strand at 734.660 kb on + strand at 734.682 kb on + strand at 734.683 kb on - strand at 734.683 kb on - strand at 734.683 kb on - strand at 734.719 kb on + strand at 734.720 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 733,046 + BPHYT_RS23050 0.19 +0.7 733,433 + -0.4 733,434 - -2.2 733,549 + +2.3 734,536 + +0.1 734,660 + +1.0 734,660 + -0.5 734,682 + +1.3 734,683 - -0.1 734,683 - -0.6 734,683 - -1.5 734,719 + -0.2 734,720 - +0.1
Or see this region's nucleotide sequence