Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23030 and BPHYT_RS23035 are separated by 367 nucleotides BPHYT_RS23035 and BPHYT_RS23040 are separated by 26 nucleotides
BPHYT_RS23030: BPHYT_RS23030 - gluconate 2-dehydrogenase, at 727,734 to 728,474
_RS23030
BPHYT_RS23035: BPHYT_RS23035 - hypothetical protein, at 728,842 to 729,639
_RS23035
BPHYT_RS23040: BPHYT_RS23040 - peptidase, at 729,666 to 731,936
_RS23040
Position (kb) 728
729
730 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 728.143 kb on + strand, within BPHYT_RS23030 at 728.436 kb on + strand at 728.456 kb on + strand at 728.457 kb on - strand at 728.482 kb on + strand at 728.483 kb on - strand at 728.483 kb on - strand at 728.585 kb on - strand at 728.819 kb on + strand at 728.819 kb on + strand at 728.820 kb on - strand at 728.820 kb on - strand at 728.904 kb on + strand at 728.946 kb on - strand, within BPHYT_RS23035 at 729.277 kb on - strand, within BPHYT_RS23035 at 729.512 kb on + strand, within BPHYT_RS23035 at 729.745 kb on + strand at 729.746 kb on - strand at 729.794 kb on - strand at 729.830 kb on + strand at 729.952 kb on + strand, within BPHYT_RS23040 at 729.953 kb on - strand, within BPHYT_RS23040 at 729.953 kb on - strand, within BPHYT_RS23040 at 730.040 kb on - strand, within BPHYT_RS23040 at 730.077 kb on - strand, within BPHYT_RS23040 at 730.077 kb on - strand, within BPHYT_RS23040 at 730.079 kb on - strand, within BPHYT_RS23040
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 728,143 + BPHYT_RS23030 0.55 -0.9 728,436 + -0.7 728,456 + +1.5 728,457 - +0.2 728,482 + -0.3 728,483 - -0.7 728,483 - -1.1 728,585 - +0.7 728,819 + +1.5 728,819 + -0.4 728,820 - +0.1 728,820 - -0.0 728,904 + +1.0 728,946 - BPHYT_RS23035 0.13 +0.2 729,277 - BPHYT_RS23035 0.55 -3.7 729,512 + BPHYT_RS23035 0.84 -3.2 729,745 + -0.2 729,746 - -1.2 729,794 - -0.5 729,830 + -0.1 729,952 + BPHYT_RS23040 0.13 -1.1 729,953 - BPHYT_RS23040 0.13 -0.3 729,953 - BPHYT_RS23040 0.13 +0.3 730,040 - BPHYT_RS23040 0.16 -2.1 730,077 - BPHYT_RS23040 0.18 -2.1 730,077 - BPHYT_RS23040 0.18 +1.5 730,079 - BPHYT_RS23040 0.18 -1.6
Or see this region's nucleotide sequence