Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22215 and BPHYT_RS22220 are separated by 7 nucleotides BPHYT_RS22220 and BPHYT_RS22225 are separated by 158 nucleotides BPHYT_RS22225 and BPHYT_RS22230 are separated by 109 nucleotides
BPHYT_RS22215: BPHYT_RS22215 - hypothetical protein, at 541,743 to 542,828
_RS22215
BPHYT_RS22220: BPHYT_RS22220 - sulfatase, at 542,836 to 544,782
_RS22220
BPHYT_RS22225: BPHYT_RS22225 - hypothetical protein, at 544,941 to 545,489
_RS22225
BPHYT_RS22230: BPHYT_RS22230 - LuxR family transcriptional regulator, at 545,599 to 546,246
_RS22230
Position (kb) 542
543
544
545 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 541.958 kb on - strand at 542.322 kb on + strand, within BPHYT_RS22215 at 542.420 kb on - strand, within BPHYT_RS22215 at 542.420 kb on - strand, within BPHYT_RS22215 at 542.788 kb on + strand at 542.886 kb on + strand at 542.887 kb on - strand at 542.888 kb on + strand at 542.888 kb on + strand at 542.889 kb on - strand at 542.889 kb on - strand at 543.159 kb on - strand, within BPHYT_RS22220 at 543.164 kb on + strand, within BPHYT_RS22220 at 543.591 kb on + strand, within BPHYT_RS22220 at 543.684 kb on - strand, within BPHYT_RS22220 at 543.762 kb on - strand, within BPHYT_RS22220 at 543.795 kb on - strand, within BPHYT_RS22220 at 543.959 kb on + strand, within BPHYT_RS22220 at 543.960 kb on - strand, within BPHYT_RS22220 at 544.062 kb on - strand, within BPHYT_RS22220 at 544.083 kb on - strand, within BPHYT_RS22220 at 544.238 kb on + strand, within BPHYT_RS22220 at 544.238 kb on + strand, within BPHYT_RS22220 at 544.238 kb on + strand, within BPHYT_RS22220 at 544.239 kb on - strand, within BPHYT_RS22220 at 544.239 kb on - strand, within BPHYT_RS22220 at 544.239 kb on - strand, within BPHYT_RS22220 at 544.242 kb on + strand, within BPHYT_RS22220 at 544.243 kb on - strand, within BPHYT_RS22220 at 544.312 kb on - strand, within BPHYT_RS22220 at 544.330 kb on - strand, within BPHYT_RS22220 at 544.331 kb on + strand, within BPHYT_RS22220 at 544.332 kb on - strand, within BPHYT_RS22220 at 544.332 kb on - strand, within BPHYT_RS22220 at 544.657 kb on + strand at 544.800 kb on - strand at 544.800 kb on - strand at 544.861 kb on + strand at 544.879 kb on + strand at 544.880 kb on - strand at 545.116 kb on + strand, within BPHYT_RS22225 at 545.117 kb on - strand, within BPHYT_RS22225 at 545.233 kb on + strand, within BPHYT_RS22225 at 545.234 kb on - strand, within BPHYT_RS22225 at 545.435 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 541,958 - +1.4 542,322 + BPHYT_RS22215 0.53 -1.2 542,420 - BPHYT_RS22215 0.62 -3.1 542,420 - BPHYT_RS22215 0.62 -1.4 542,788 + +0.7 542,886 + -0.5 542,887 - -0.6 542,888 + +0.5 542,888 + -2.4 542,889 - +0.2 542,889 - -2.2 543,159 - BPHYT_RS22220 0.17 -0.3 543,164 + BPHYT_RS22220 0.17 -0.8 543,591 + BPHYT_RS22220 0.39 -0.3 543,684 - BPHYT_RS22220 0.44 +0.9 543,762 - BPHYT_RS22220 0.48 -2.4 543,795 - BPHYT_RS22220 0.49 +0.7 543,959 + BPHYT_RS22220 0.58 -0.9 543,960 - BPHYT_RS22220 0.58 -0.6 544,062 - BPHYT_RS22220 0.63 +0.5 544,083 - BPHYT_RS22220 0.64 +0.6 544,238 + BPHYT_RS22220 0.72 -0.1 544,238 + BPHYT_RS22220 0.72 -2.1 544,238 + BPHYT_RS22220 0.72 -0.6 544,239 - BPHYT_RS22220 0.72 -1.2 544,239 - BPHYT_RS22220 0.72 +0.3 544,239 - BPHYT_RS22220 0.72 +0.1 544,242 + BPHYT_RS22220 0.72 +0.4 544,243 - BPHYT_RS22220 0.72 -0.4 544,312 - BPHYT_RS22220 0.76 +0.5 544,330 - BPHYT_RS22220 0.77 -0.6 544,331 + BPHYT_RS22220 0.77 -1.8 544,332 - BPHYT_RS22220 0.77 -0.0 544,332 - BPHYT_RS22220 0.77 -1.4 544,657 + -2.4 544,800 - +0.3 544,800 - -0.9 544,861 + -1.2 544,879 + +1.1 544,880 - -0.2 545,116 + BPHYT_RS22225 0.32 -0.2 545,117 - BPHYT_RS22225 0.32 -0.5 545,233 + BPHYT_RS22225 0.53 -2.6 545,234 - BPHYT_RS22225 0.53 -0.3 545,435 + +0.4
Or see this region's nucleotide sequence