Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22205 and BPHYT_RS22210 overlap by 7 nucleotides BPHYT_RS22210 and BPHYT_RS22215 overlap by 4 nucleotides BPHYT_RS22215 and BPHYT_RS22220 are separated by 7 nucleotides
BPHYT_RS22205: BPHYT_RS22205 - hypothetical protein, at 540,002 to 540,958
_RS22205
BPHYT_RS22210: BPHYT_RS22210 - metal ABC transporter substrate-binding protein, at 540,952 to 541,746
_RS22210
BPHYT_RS22215: BPHYT_RS22215 - hypothetical protein, at 541,743 to 542,828
_RS22215
BPHYT_RS22220: BPHYT_RS22220 - sulfatase, at 542,836 to 544,782
_RS22220
Position (kb) 541
542
543 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 540.908 kb on - strand at 541.958 kb on - strand at 542.322 kb on + strand, within BPHYT_RS22215 at 542.420 kb on - strand, within BPHYT_RS22215 at 542.420 kb on - strand, within BPHYT_RS22215 at 542.788 kb on + strand at 542.886 kb on + strand at 542.887 kb on - strand at 542.888 kb on + strand at 542.888 kb on + strand at 542.889 kb on - strand at 542.889 kb on - strand at 543.159 kb on - strand, within BPHYT_RS22220 at 543.164 kb on + strand, within BPHYT_RS22220 at 543.591 kb on + strand, within BPHYT_RS22220 at 543.684 kb on - strand, within BPHYT_RS22220 at 543.762 kb on - strand, within BPHYT_RS22220 at 543.795 kb on - strand, within BPHYT_RS22220
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 540,908 - +0.0 541,958 - +1.4 542,322 + BPHYT_RS22215 0.53 -1.2 542,420 - BPHYT_RS22215 0.62 -3.1 542,420 - BPHYT_RS22215 0.62 -1.4 542,788 + +0.7 542,886 + -0.5 542,887 - -0.6 542,888 + +0.5 542,888 + -2.4 542,889 - +0.2 542,889 - -2.2 543,159 - BPHYT_RS22220 0.17 -0.3 543,164 + BPHYT_RS22220 0.17 -0.8 543,591 + BPHYT_RS22220 0.39 -0.3 543,684 - BPHYT_RS22220 0.44 +0.9 543,762 - BPHYT_RS22220 0.48 -2.4 543,795 - BPHYT_RS22220 0.49 +0.7
Or see this region's nucleotide sequence