Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21625 and BPHYT_RS21630 are separated by 184 nucleotides BPHYT_RS21630 and BPHYT_RS21635 are separated by 203 nucleotides BPHYT_RS21635 and BPHYT_RS21640 are separated by 41 nucleotides
BPHYT_RS21625: BPHYT_RS21625 - LysR family transcriptional regulator, at 407,235 to 408,149
_RS21625
BPHYT_RS21630: BPHYT_RS21630 - NmrA family transcriptional regulator, at 408,334 to 409,197
_RS21630
BPHYT_RS21635: BPHYT_RS21635 - extradiol dioxygenase, at 409,401 to 409,805
_RS21635
BPHYT_RS21640: BPHYT_RS21640 - alpha/beta hydrolase, at 409,847 to 410,698
_RS21640
Position (kb) 408
409
410 Strain fitness (log2 ratio) -1
0
1
2 at 407.623 kb on + strand, within BPHYT_RS21625 at 408.109 kb on - strand at 408.215 kb on - strand at 408.308 kb on - strand at 408.308 kb on - strand at 408.311 kb on + strand at 408.312 kb on - strand at 408.315 kb on + strand at 408.315 kb on + strand at 409.053 kb on + strand, within BPHYT_RS21630 at 409.344 kb on - strand at 409.344 kb on - strand at 409.493 kb on - strand, within BPHYT_RS21635 at 409.500 kb on - strand, within BPHYT_RS21635 at 409.783 kb on - strand at 409.803 kb on + strand at 409.804 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 407,623 + BPHYT_RS21625 0.42 -0.9 408,109 - +2.1 408,215 - -1.2 408,308 - -1.3 408,308 - +0.9 408,311 + -0.1 408,312 - +0.1 408,315 + -1.0 408,315 + -0.4 409,053 + BPHYT_RS21630 0.83 +0.1 409,344 - -1.3 409,344 - -1.3 409,493 - BPHYT_RS21635 0.23 -0.2 409,500 - BPHYT_RS21635 0.24 +0.8 409,783 - +0.5 409,803 + -0.3 409,804 - -0.0
Or see this region's nucleotide sequence