Experiment: P. phyto PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20590 and BPHYT_RS20595 are separated by 162 nucleotides BPHYT_RS20595 and BPHYT_RS20600 are separated by 85 nucleotides BPHYT_RS20600 and BPHYT_RS20605 are separated by 279 nucleotides
BPHYT_RS20590: BPHYT_RS20590 - methionine sulfoxide reductase A, at 179,969 to 180,475
_RS20590
BPHYT_RS20595: BPHYT_RS20595 - hypothetical protein, at 180,638 to 180,910
_RS20595
BPHYT_RS20600: BPHYT_RS20600 - peroxiredoxin, at 180,996 to 181,421
_RS20600
BPHYT_RS20605: BPHYT_RS20605 - LysR family transcriptional regulator, at 181,701 to 182,837
_RS20605
Position (kb) 180
181
182 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 180.372 kb on + strand, within BPHYT_RS20590 at 180.373 kb on - strand, within BPHYT_RS20590 at 180.373 kb on - strand, within BPHYT_RS20590 at 180.390 kb on - strand, within BPHYT_RS20590 at 180.721 kb on - strand, within BPHYT_RS20595 at 180.816 kb on + strand, within BPHYT_RS20595 at 181.012 kb on - strand at 181.240 kb on + strand, within BPHYT_RS20600 at 181.240 kb on + strand, within BPHYT_RS20600 at 181.240 kb on + strand, within BPHYT_RS20600 at 181.241 kb on - strand, within BPHYT_RS20600 at 181.652 kb on - strand at 181.656 kb on + strand at 181.657 kb on - strand at 181.657 kb on - strand at 181.664 kb on - strand at 181.683 kb on + strand at 181.684 kb on - strand at 181.805 kb on - strand at 182.204 kb on - strand, within BPHYT_RS20605 at 182.227 kb on - strand, within BPHYT_RS20605
Per-strain Table
Position Strand Gene LocusTag Fraction P. phyto PHYTO2 MOI 1 remove 180,372 + BPHYT_RS20590 0.79 -2.7 180,373 - BPHYT_RS20590 0.80 -0.6 180,373 - BPHYT_RS20590 0.80 -2.6 180,390 - BPHYT_RS20590 0.83 -0.8 180,721 - BPHYT_RS20595 0.30 +0.0 180,816 + BPHYT_RS20595 0.65 -0.4 181,012 - -1.5 181,240 + BPHYT_RS20600 0.57 -0.6 181,240 + BPHYT_RS20600 0.57 +0.2 181,240 + BPHYT_RS20600 0.57 -2.8 181,241 - BPHYT_RS20600 0.58 -1.6 181,652 - +0.5 181,656 + -0.6 181,657 - -0.4 181,657 - -0.7 181,664 - +0.1 181,683 + -1.2 181,684 - +1.5 181,805 - -0.3 182,204 - BPHYT_RS20605 0.44 -0.1 182,227 - BPHYT_RS20605 0.46 -1.5
Or see this region's nucleotide sequence